1st Edition

Martin Latterich

Taylor & Francis
Published November 8, 2007
Reference - 150 Pages
ISBN 9780415409506 - CAT# RU9500Z
Series: Advanced Methods


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  • Provides a compilation of RNAi techniques and their common applications that can be accessed by novices and experts alike.
  • Encourages further scientific thought, experimentation and development, due to the relatively 'new' nature of the field.
  • Covers both general and specialized uses of RNAi.
  • Summary

    One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful research tool in the functional study of individual genes and their products, and in functional genomics. In RNAi, specific small double-stranded RNA molecules (small interfering RNAs or siRNAs) are introduced into cells to selectively silence certain genes.

    RNAi covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent.  A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations.

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