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Advanced Methods


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RNAi

RNAi

Martin Latterich
November 08, 2007

One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful...

Real-time PCR

Real-time PCR

M Tevfik Dorak
April 27, 2006

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the...

DNA Microarrays

DNA Microarrays

Ulrike Nuber
October 20, 2005

DNA Microarrays introduces all up-to-date microarray platforms and their various applications.  It is written for scientists who are entering the field of DNA microarrays as well as those already familiar with the technology, but interested in new applications and methods....

Molecular Microbial Ecology

Molecular Microbial Ecology

Mark Osborn, Cindy Smith
August 11, 2005

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of...

Gene Transfer to Animal Cells

Gene Transfer to Animal Cells

Richard Twyman
December 23, 2004

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes...

Gene Targeting and Embryonic Stem Cells

Gene Targeting and Embryonic Stem Cells

Jim Mcwhir, Alison Thomson
July 01, 2004

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines.  Gene targeting...

Cell Proliferation and Apoptosis

Cell Proliferation and Apoptosis

David Hughes, Huseyin Mehmet
May 22, 2003

Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation...

Microarrays and Microplates: Applications in Biomedical Sciences

Microarrays and Microplates: Applications in Biomedical Sciences

Ian Day, S Ye
August 01, 2002

The 'Advanced Methods' series is intended for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to...

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