Optical Probes in Biology

Jin Zhang, Sohum Mehta, Carsten Schultz

November 30, 2017 by CRC Press
Reference - 511 Pages - 85 B/W Illustrations
ISBN 9781138199934 - CAT# K31525
Series: Series in Cellular and Clinical Imaging

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Features

  • Focuses on the use of optical probes to track events in single cells and living animals, including current in vivo imaging approaches
  • Describes the properties of fluorescent proteins and their importance in the design of fluorescent biosensors
  • Explains how Förster/fluorescence resonance energy transfer (FRET)-based biosensors report key molecular information
  • Helps readers choose the best probe design for their application
  • Provides detailed discussions on selecting the appropriate imaging experiment and experimental set-up for answering a given biological question
  • Covers several promising new techniques, including super-resolution microscopy that can observe biological phenomena in unprecedented spatial detail as well as optogenetic techniques that can both detect and manipulate intracellular events

Summary

Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them.

The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes.

In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers.

With contributions from top international scientists, this book offers a thorough overview of the latest optical probes in cell biology and biochemistry. Both newcomers and established researchers will discover how to incorporate state-of-the-art optical probes and fluorescence imaging into their research.

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