Since the first successful isolation and cultivation of human embryonic stem cells at the University of Wisconsin, Madison in 1998, there has been high levels of both interest and controversy in this area of research.This book provides a concise overview of an exciting field, covering the characteristics of both human embryonic stem cells and pluripotent stem cells from other human cell lineages. The following chapters describe state-of-the-art differentiation and characterization of specific ectoderm, mesoderm and endoderm-derived lineages from human embryonic stem cells, emphasizing how these can be used to study human developmental mechanisms. A further chapter discusses genetic manipulation of human ES cells. The concluding section covers therapeutic applications of human ES cells, as well as addressing the ethical and legal issues that this research have raised.
Table of Contents
1. Biology of Embryonic Stem Cells 2. Characteristics of Human Embryonic Stem Cells, Embryonal Carcinoma Cells and Embryonic Germ Cells 3. Adult Stem Cell Plasticity 4. Human and Murine Embryonic Stem Cell Lines: Windows to Early Mammalian Development 5. Human Mesenchymal Stem Cells and Multilineage Differentiation to Mesoderm Lineages 6. Trophoblast Differentiation from Embryonic Stem Cells 7. Current and Future Prospects for Hematopoiesis Studies using Human Embryonic Stem Cells 8. Derivation of Endothelial Cells from Human Embryonic Stem Cells 9. Neural Specification from Human Embryonic Stem Cells 10. Modeling Islet Development through Embryonic Stem Cell Differentiation 11. Cardiomyocyte Differentiation in Human Embryonic Stem Cell Progeny 12. Genetic Engineering of Human Embryonic Stem Cells 13. ES Cells for Transplantation: Coping with Immunity 14. Clinical Applications for Human ES Cells 15. Production of Human Embryonic Stem Cell Derived Cellular Product for Therapeutic Use 16. Ethical and Policy Considerations in Embryonic Stem Cell Research 17. Legal Framework Pertaining to Research Creating or Using Human Embryonic Stem Cells 18. Genomic Approaches to Stem Cell Biology 18. Genomic Approaches to Stem Cell Biology 19. Proteomics and Embryonic Stem Cells
The book provides an outstanding introduction to embryonic stem cell research...The book includes an excellent mix of photomicrographs and diagrams as well as a few color plates...This is a well-written and easy-to-read, compact book. The editors have brought together outstanding experts and assembled a nice cross-sectional view of this enlarging field - Doody's Listings and Reviews
This book will be of tremendous help to those who have already started, or soon will start, a journey into the murky waters of stem-cell research - The New England Journal of Medicine, 354: 1, Jan 06