1st Edition

High Performance Liquid Chromatography in Phytochemical Analysis

Edited By Monika Waksmundzka-Hajnos, Joseph Sherma Copyright 2011
    996 Pages 281 B/W Illustrations
    by CRC Press

    The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field.

    The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products.

    Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

    PART I
    Overview of the Field of Column High Performance Liquid Chromatography in Phytochemical Analysis and the Structure of the Book, Monika Waksmundzka-Hajnos and Joseph Sherma

    Herbal Drugs and the Role of Chromatographic Methods in Their Analysis, Ioanna Chinou

    Plant Products in Nutrition and Dietary Supplements: Quality Control, Grażyna Zgórka

    HPLC in Chemosystematics, Renate Spitaler and Christian Zidorn

    Phytochemistry, Phytopharmacology, and the Biological Role of Plant Metabolites, Krystyna Skalicka-Woźniak, Michał Hanos, and Kazimierz Głowniak

    Sample Preparation of Plant Material, Anna Oniszczuk and Anna Hawrył

    Stationary Phases and Columns in Analysis of Primary and Secondary Metabolites, Fred Rabel

    Separation of Nonionic Analytes: Reversed- and Normal-Phase HPLC, Mirosław Hawrył and Wojciech Markowski

    Separation of Ionic Analytes: Reversed-Phase, Ion-Pair, Ion-Exchange, and Ion-Exclusion HPLC, Monika Waksmundzka-Hajnos and Łukasz Cieśla

    Gradient Elution and Computer-Assisted Method Development, Mirosław Hawrył

    LC-MS as a Method of Identification and Quantification of Plant Metabolites, Gunther Stecher, Robert Mayer, Thomas Ringer, Muhammad A. Hashir, Saowapak Kasemsook, Muhammad N. Qureshi, and Gunther K. Bonn

    LC-NMR and Related Techniques for the Rapid Identification of Plant Metabolites, Jean-Luc Wolfender

    Photodiode Array (PDA) and Other Detection Methods in HPLC of Plant Metabolites, Wojciech Markowski and Monika Waksmundzka-Hajnos

    Quantitative Analysis—Method Validation—Quality Control, Pierre Masson

    Confirmation of Chirality of Some Natural Products by the HPLC Method, Beata Polak

    PART II
    Primary Metabolites

    HPLC of Carbohydrates, Angelika Koch, Simla Basar, and Rita Richter

    HPLC of Plant Lipids, Marek Gołębiowski, Monika Paszkiewicz, Łukasz Haliński, and Piotr Stepnowski

    HPLC Analysis of Amino Acids, Peptides, and Proteins, Giuseppe Mennella, Antonietta D’Alessandro, and Gianluca Francese

    Secondary Metabolites—Shikimic Acid Derivatives
    Application of HPLC in the Analysis of Phenols, Phenolic Acids, and Tannins, Tuulia Hyotylainen and Maarit Kivilompolo

    Application of HPLC in Coumarin Analyses, Alev Tosun and Petr Tomek

    High Performance Liquid Chromatography of Flavonoids, Monika Waksmundzka-Hajnos, Anna Oniszczuk, Mieczysław Hajnos, and Tomasz Oniszczuk

    HPLC of Lignans, Annika I. Smeds

    Secondary Metabolites—Isoprenoids
    HPLC of Mono- and Sesquiterpenes, Angelika Koch, Simla Basar, and Rita Richter

    HPLC Analysis of Diterpenes, Michał Ł. Hajnos

    High Performance Liquid Chromatography of Triterpenes (Including Saponins), Wieslaw Oleszek and Anna Stochmal

    HPLC of Carotenoids, Łukasz Cieśla

    HPLC of Steroids, Laurence Dinan, Juraj Harmatha, and René Lafont

    HPLC of Iridoids, Rilka Taskova, Tetsuo Kokubun, and Kalina Alipieva

    Secondary Metabolites—Amino Acid Derivatives
    HPLC of Indole Alkaloids, Anna Petruczynik

    HPLC of Isoquinoline Alkaloids, László Kursinszki, Hajnalka Hank, Ágnes Kéry, and Éva Szőke

    HPLC of Tropane Alkaloids, Tomasz Mroczek

    HPLC of Alkaloids from the Other Biosynthetic Groups, Jolanta Flieger

    Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)
    HPLC Analysis of Polyacetylenes, Lars P. Christensen and Kathrine B. Christensen

    HPLC of Quinonoid Phytochemicals, Subhalakshmi Ghosh, Madhushree Das Sarma, and Banasri Hazra

    Index

    Biography

    Monika Waksmundzka-Hajnos is a professor in the Department of Inorganic Chemistry at the Medical University of Lublin in Poland.

    Joseph Sherma is the John D. and Frances H. Larkin Professor Emeritus of Chemistry in the Department of Chemistry at Lafayette College in Easton, Pennsylvania, USA.