While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled with the fact that 60 percent of current drugs are based on targeting this group of proteins, a trend that does not seem likely to reverse.
Structural Genomics on Membrane Proteins provides an excellent overview on novel research in bioinformatics and modeling on membranes, as well as the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins. This cutting-edge work also explains the difficulties facing researchers—both technical and ethical—that have slowed the process.
Structural Genomics on Membrane Proteins provides researchers with an unprecedented look at the novel technologies that will ultimately allow them to conquer the last frontier in structural biology, leading to accelerated breakthroughs in drug discovery.
Introduction; Kenneth Lundstrom
Bioinformatics in Membrane Protein Analysis; Bengt Persson
Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification; Joanne Clough, Massoud Saidijam, Kim Bettaney, Gerda Szakonyi, Simon Patching, Johan Meuller, Shun’ichi Suzuki, Keigo Shibayama, Mark Bacon, Emma Barksby, Marie Groves, Richard Herbert, Mary Phillips-Jones, Alison Ward, Frank Gunn-Moore, John O’Reilly, Nick Rutherford, Roslyn Bill, and Peter Henderson
Membrane Protein Production Strategies for Structural Genomics; Said Eshaghi and Pär Nordlund
Refolding of Membrane Proteins for Large-Scale Production; Hans Kiefer, Thomas Ostermann, and Monika Bähner
Crystallization of Membrane Proteins; Alastair T. Gardiner, June Southall, and Richard J. Cogdell
Signaling through Membrane Proteins; J. Robert Bostwick and Deborah S. Hartman
Expression of Membrane Proteins in Yeasts; Christoph Reinhart and Christoph Krettler
Expression of Functional Membrane Proteins in the Baculovirus–Insect Cell System: Challenges and Developments; Giel J.C.G.M. Bosman and Willem J. De Grip
Expression of Membrane Proteins in Mammalian Cells; Kenneth Lundstrom
Solubilization and Purification of Membrane Proteins; Bernadette Byrne and Mika Jormakka
Fluorescent Labelling of Membrane Proteins in Living Cells; Ruud Hovius, Bruno H. Meyer, Emmanuel G. Guignet, and Horst Vogel
Membrane Protein NMR; Xiang-Qun (Sean) Xie
Miniaturization of Structural Biology Technologies — From Expression to Biophysical Analyses; Enrique Abola, Peter Kuhn and Raymond C. Stevens
Electron Microscopy and Atomic Force Microscopy of Reconstituted Membrane Proteins; Andreas Engel
Structural Genomics Networks for Membrane Proteins; Kenneth Lundstrom
Molecular Modelling of Membrane Proteins; Slawomir Filipek and Anna Modzelewska
Toward Structural Bases for GPCR Ligand binding: A Path for Drug Discovery; Geoffrey F. Stamper, Sabrina A. Beretta, Alla Korepanova, Mulugeta Mamo, and Mark L. Chiu
“This volume informs the actual state of the investigation of membrane’s proteins … The editor has selected a good group of specialists that update the aspects of interest about the ways to follow this direction. … With this edition, researchers will be able to obtain valuable information that will allow them to advance in the knowledge of the structure of the integral proteins of the membrane, so important for the cellular’s functions.”
— Journal of Physiology and Biochemistry, Vol. 62, No. 3, September 2006
“… provides the reader with an overview of structural biology research and recent technological developments relating to membrane proteins. With contributions from more than 40 researches, this volume serves as a useful reference for background and methodologies. The various approaches for recombinant expression and production of membrane proteins in bacteria, yeast, insect and mammalian cells are reviewed. … This is a recommended reference for research laboratories involved in the study and expression of recombinant proteins.”
— Zehra Elgundi, research assistant at the Garvan Institute and a budding science writer, Australian Life Scientist
"… provides insight into the various disciplines, approaches, problems, and solutions in membrane protein research. Clearly, it is a challenge to cover all aspects of this research area, but the editor and authors have achieved a balance between detail and importance without losing the main focus. … recommended for experts in membrane protein research or structural genomics because it not only provides an excellent overview, it also offers a detailed and balanced view on the daily challenges in working with these fascinating proteins, especially from a structural genomics perspective."
— Lutz Schmitt, Heinrich Heine University, Germany in CHEMMEDCHEM, 2008