Methods for Studying Nucleic Acid/Drug Interactions

Meni Wanunu, Yitzhak Tor

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December 20, 2011 by CRC Press
Reference - 392 Pages - 154 B/W Illustrations
ISBN 9781439839737 - CAT# K11829

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Features

  • Inspires young scientists to continue the advancement of these methods in light of current capabilities for assay miniaturization and enhanced sensitivity using microfluidics and nanomaterials
  • Surveys possible future techniques as well as highlights their drawbacks and advantages with respect to commonly used tools
  • Introduces several classical techniques, including crystallography, NMR and mass spectroscopy, optical, and calorimetry-based techniques
  • Details diverse aspects of powerful fluorescence-based techniques, electrospray mass spectrometry (ESI-MS) techniques, electrophoresis-based techniques, surface plasmon resonance (SPR) detection, continuous wave (CW) EP, computational modeling, and microarray-based methods
  • Reports on the use of optical tweezers, atomic force microscopy (AFM), and the use of nanopores as ion microscopes that scan individual nucleic acids and detect ligand binding
  • Discusses theoretical developments useful for analyzing and interpreting experimental data, including chemical kinetics, a theoretical perspective on DNA/drug interactions and a wide array of molecular dynamics studies that investigate the interactions of various ligands with nucleic acids

Summary

Since most therapeutic efforts have been predominantly focused on pharmaceuticals that target proteins, there is an unmet need to develop drugs that intercept cellular pathways that critically involve nucleic acids. Progress in the discovery of nucleic acid binding drugs naturally relies on the availability of analytical methods that assess the efficacy and nature of interactions between nucleic acids and their putative ligands. This progress can benefit tremendously from new methods that probe nucleic acid/ligand interactions both rapidly and quantitatively.

A variety of novel methods for these studies have emerged in recent years, and Methods for Studying DNA/Drug Interactions highlights new and non-conventional methods for exploring nucleic acid/ligand interactions. Designed to present drug-developing companies with a survey of possible future techniques, the book compares their drawbacks and advantages with respect to commonly used tools. Perhaps more importantly, this book was written to inspire young scientists to continue to advance these methods into fruition, especially in light of current capabilities for assay miniaturization and enhanced sensitivity using microfluidics and nanomaterials.

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