1st Edition

Immunology in Plant Health and Its Impact on Food Safety

By P. Narayanasamy Copyright 2005
    416 Pages
    by CRC Press

    416 Pages
    by CRC Press

    Find out more about convenient immunoassays you can implement in your own research!

    From the Foreword, by M. S. Swaminathan, Chairman of the M. S. Swaminathan Research Foundation: “The book provides remedies to the common maladies relating to quality and safety of dietary material. Professor Narayanasamy has compiled and presented with great clarity the latest information on all aspects relating to immunology in plant health and food safety. We owe Professor Narayanasamy a deep debt of gratitude for this labor of love in the cause of improving food and feed quality and safety.”

    Immunology in Plant Health and Its Impact on Food Safety suggests cost-effective, simple, and sensitive immunological techniques to assess plant health and food safety for the production of desirable foods, feeds, and timbers. This book explores the structure and biochemical constituents of healthy plants and the abiotic and biotic stresses that can cause a marked reduction in quantity and quality of agricultural produce. Researchers, faculty members, and graduate scholars in plant pathology, microbiology, biochemistry, environmental sciences, and food technology will find this text useful for producing healthy plants while maintaining a pollution-free environment.

    In Immunology in Plant Health and Its Impact on Food Safety, methods to develop stress-resistant cultivars are discussed to enable you to select the most suitable strategies for maintaining production and quality without the use of chemicals. This valuable resource provides detailed instructions for employing immunoassays that are rapid, reproducible, and amenable for large-scale application in place of cumbersome and expensive methods currently in use. With this important tool, you will be able to plan and develop programs to obtain agricultural produce of high quality acceptable for human and animal consumption.

    With Immunology in Plant Health and Its Impact on Food Safety, you’ll learn more about:

    • agrosystems
    • immunological reactions
    • preparations of antisera
    • immunodetection techniques
    • plant-stress interactions
    • genetic manipulations
    • disease resistance and the production of disease-free plants
    • mycotoxins
    • chemical residues
    This essential guide provides you with access to a wide spectrum of information never before encompassed in a single book, saving you time and energy. Figures, photographs, and tables with appropriate data supply visual and factual support for the points discussed in the text. Immunology in Plant Health and Its Impact on Food Safety includes a large number of citations (over 1000) for further research and development in your chosen field of study.

    • Foreword (M. S. Swaminathan)
    • Preface
    • Acknowledgments
    • List of Abbreviations
    • PART I: PRINCIPLES AND TECHNIQUES
    • Chapter 1. Introduction
    • Agroecosystems and Microbes
    • Plant Health Management
    • Applications of Immunology
    • Summary
    • Chapter 2. Principles of Immunological Reactions
    • Antigens
    • Antibodies
    • Summary
    • Chapter 3. Preparation of Antisera
    • Polyclonal Antisera
    • Monoclonal Antisera
    • Phage-Displayed Recombinant Antibodies
    • Purification of Immunoglobulins and Antibody Fragments
    • Summary
    • Chapter 4. Basic Immunodetection Techniques
    • Precipitation and Agglutination Reactions
    • Immunodiffusion Tests
    • Immunoelectrophoresis
    • Labeled Antibody Techniques
    • Immunoelectron Microscopy
    • Immunocapture-Reverse Transcription-Polymerase Chain Reaction (IC-RT-PCR) Technique
    • Immunocapillary Zone Electrophoresis (I-CZE)
    • Summary
    • PART II: STRUCTURE AND CONSTITUENTS OF HEALTHY PLANTS
    • Chapter 5. Structure and Functions of Plant Components
    • Components of Plant Cells
    • Organization of Tissues of the Plant Body
    • Summary
    • Chapter 6. Biochemical Constituents of Plants
    • Carbohydrates
    • Proteins
    • Nucleic Acids
    • Summary
    • Appendix: Assay of Polygalacturonase-Inhibiting Protein (PGIP) by Immunoblotting Technique
    • PART III: PLANT-STRESS INTERACTIONS
    • Chapter 7. Plant-Abiotic Stress Interactions
    • Salt Stress
    • Water Stress
    • Temperature Stress
    • Summary
    • Chapter 8. Plant-Fungal Pathogen Interactions
    • Immunodiagnostic Techniques
    • Enzyme-Linked Immunosorbent Assay (ELISA)
    • Phases of Fungal Disease Development
    • Summary
    • Appendix: Immunological Protocols for Detection of Fungal Pathogens
    • Chapter 9. Plant-Bacterial Pathogen Interactions
    • Immunodiagnostic Techniques for Bacterial Pathogens
    • Immunodiagnosis of Phytoplasmal Diseases
    • Phases of Development of Bacterial Diseases
    • Summary
    • Appendix: Diagnostic Techniques for the Detection of Bacterial Pathogens
    • Chapter 10. Plant-Viral Pathogen Interactions
    • Immunodiagnostic Techniques for Plant Viruses
    • Virus Disease Development
    • Summary
    • Appendix: Techniques for Detection of Viral Pathogens
    • PART IV: APPROACHES FOR PLANT HEALTH MANAGEMENT
    • Chapter 11. Improvement of Host Plant Resistance Through Genetic Manipulation
    • Breeding for Resistance to Crop Diseases
    • Engineering Resistance to Crop Diseases
    • Summary
    • Appendix: Quantification of Infection and Detection by Immunological Techniques
    • Chapter 12. Induction of Disease Resistance and Production of Disease-Free Plants
    • Induction of Resistance to Microbial Plant Pathogens
    • Cross Protection
    • Production of Disease-Free Plants
    • Summary
    • Appendix: Protocol for Polyclonal Antiserum Production
    • PART V: ASSESSMENT OF FOOD SAFETY
    • Chapter 13. Mycotoxins and Food Safety
    • Mycotoxin Contamination
    • Detection of Mycotoxins
    • Management of Mycotoxin Hazards
    • Summary
    • Appendix: Protocols for the Detection of Mycotoxins
    • Chapter 14. Chemical Residues and Food Safety
    • Detection of Fungicides
    • Detection of Pesticides Applied Against Insect Vectors
    • Detection of Predation of Crop Pests
    • Summary
    • Appendix: Immunoassay Protocols
    • References
    • Index

    Biography

    P. Narayanasamy