1st Edition

The Metabolic Pathway Engineering Handbook Tools and Applications

Edited By Christina Smolke Copyright 2009
    582 Pages 15 Color & 124 B/W Illustrations
    by CRC Press

    582 Pages 15 Color & 124 B/W Illustrations
    by CRC Press

    This second volume of the Metabolic Pathway Engineering Handbook delves into evolutionary tools and gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels.

    Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.

    Volume II

    Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert

    Evolutionary engineering of industrially important microbial phenotypes

    Expanding enzyme function and activity through molecular evolution

    Evolving the function of DNA and RNA regulatory regions through molecular evolution

    Evolving pathways and genomes for the production of natural and novel compounds

    Models predicting optimized evolutionary strategies

    Section VII. Gene Expression Tools for Metabolic Pathway Engineering, Christina Smolke

    Low-copy number plasmids as artificial chromosomes

    Chromosomal engineering strategies

    Regulating gene expression through engineered RNA technologies

    Tools designed to regulate translational efficiency

    Regulating gene expression through post-translational modifications

    Engineering bifunctional enzymes for optimized metabolite transfer between sequential conversion steps

    Practical pathway engineering – demonstration in integrating tools

    Section VIII. Applications of Emerging Technologies to Metabolic Engineering, Jay Keasling

    Genome-wide technologies: DNA and phenotypic microarrays, proteomics

    Monitoring and measuring the metabolome

    Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee

    Genome-scale models, systems biology, and metabolic engineering

    Cell-free systems for metabolic engineering

    Dynamic in silico cell models

    Appendix I: Tools for Experimentally Determining Flux through Pathways, Ralf Takors

    Measuring flux by isotope labeling

    Tools for measuring intermediate and product formation

    Quantitative analysis of labeling data

    Appendix II: Applications, Brian Pfleger and Brian Brazeau

    Cofactor regulation in biocatalytic redox reactions

    Metabolic engineering for fine chemical production

    Metabolic engineering for drug production

    Metabolic engineering for alternative fuels

    Biography

    Christina Smolke