1st Edition

Analysis of Growth Factor Signaling in Embryos

Edited By Malcolm Whitman, Amy Sater Copyright 2007
    435 Pages 14 Color & 32 B/W Illustrations
    by CRC Press

    Developmental biologists have been driven to investigate growth factor signaling in embryos in order to understand the regulatory mechanisms underlying a given developmental process. Thus, it is critical to explore the technical methods and experimental designs for growth factor signaling in embryos.

    Focusing on specific pathways or pathway components, Analysis of Growth Factor Signaling in Embryos provides the methods and guidelines for experimental design to study major aspects of cell signaling in vertebrate embryos. The book covers a broad range of topics in signaling and a variety of current model organisms. Section I explores specific signaling pathways or pathway components. In this section, some chapters highlight the biochemistry of signaling pathways during development, which is often distinctive from that observed in cell culture systems. Section II discusses ionic regulatory mechanisms and the two chapters in Section III examine ways of investigating gene regulation in response to extracellular signals. Finally, Section IV addresses emerging strategies that facilitate integrated analyses of cell signaling in vivo in embryonic systems.

    Featuring contributions from expert researchers, Analysis of Growth Factor Signaling in Embryos will provide a foundation for further explorations of the cellular regulatory mechanisms governing vertebrate embryonic development.

    SIGNALS AND PATHWAYS
    Analysis of Canonical Wnt Signaling in Xenopus Embryos
    Wilson K. Clements and David Kimelman
    How to Assay Non-Canonical Wnt Signaling: A Critical Analysis
    Michael Kühl and Randall T. Moon
    Regulation of TGF-β Family Activity by Proprotein Processing
    Shailaja Sopory and Jan L. Christian
    Analysis of MAP Kinase Pathways in Vertebrate Development
    Amy K. Sater and Heithem M. El-Hodiri
    Analysis of Retinoid Signalling in Embryos
    Malcolm Maden
    Wnt Signaling via the Rho Family of GTPases during Embryonic Development
    Raymond Habas and Xi He
    Phosphospecific Antibodies as Tools for the Study of Signal
    Transduction during Development
    Malcolm Whitman
    IONIC SIGNALS
    Dynamic Analysis of Calcium Signaling in Animal Development
    Diane C. Slusarski
    Strategies and Techniques for Investigation of Biophysical Signals in Patterning
    D.S. Adams and M. Levin
    TRANSCRIPTIONAL REGULATION OF TARGET GENES
    Expression Profiling in Xenopus Embryos
    Curtis Altmann
    Chromatin Immunoprecipitation for in vivo Studies of
    Transcriptional Regulation during Development
    Daniel R. Buchholz, Bindu D. Paul, and Yun-Bo Shi
    EMERGING STRATEGIES FOR THE ANALYSIS OF SIGNALING IN DEVELOPMENT
    Chemical Biology in Zebrafish Vascular Development
    Joanne Chan and Thomas M. Roberts
    Investigating Gastrulation
    Karen Symes
    Bringing Small Molecule Regulation of Protein Activity to
    Developmental Systems
    Karen J. Liu, Jason E. Gestwicki, and Gerald R. Crabtree
    Systems Analysis of Signaling Pathways
    Gregory R. Hoffman, Kevin Brown, Adrian Salic, and Ethan Lee
    INDEX

    Biography

    Malcolm Whitman, Amy Sater