Reviews basic principles of micromachining and fluid flow and control for lab-on-a-chip technologyEmphasizes analytical applications of microfluidic technologyIncludes information key to cell, nucleic acid, protein, and genetic analyses, including DNA sequencingAdapts traditional principles of operations for the size of a chip, optimizing the miniaturization advantage and favorable scaling downGleans significant and detailed references from the vast literature on each topic for the more experienced researcherContains figures that represent novel designs and breakthroughs in the field, tables that summarize related materials, and problem sets that test comprehension of the material
The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information.
Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays.
Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.
Table of Contents
Micromachining of Silicon
Micromachining of Glass
Micromachining of Fused Quartz (or Fused Silica)
Micromachining of Polymeric Chips
Liquid Pumping Methods
Microfluidic Flow Control
Other Sample Injection Methods
Other Pre-concentration Methods
Gas Chromatography (GC)
Capillary Electrophoresis (CE)
Optical Detection Methods
Electrochemical (EC) Detection
Mass Spectrometry (MS)
Other Detection Methods
Applications to Cellular/Particle Analysis
Retention of Cells and Particles
Studies of Cells in a Flow
Other Cell Operations
Applications to Nucleic Acids Analysis
Nucleic Acids Extraction and Purification
Nucleic Acids Amplification
Other Nucleic Acid Applications
Applications to Protein Analysis
Nucleic Acid Analysis
"…truly an awesome book…This well-documented book with a multitude of references: 1/178 references are integrated throughout the book…well-written and compares well to other science books of its kind…a great book to learn about instrument design, with numerous pictures and illustrations making the text much easier to understand. Good pictures are something that is lacking in all too many science books."
-Gary S. Vardon, Salt Lake Tribune, in Journal of Materials
"In fact I can say that it is an excellent book, well thought out and written."
– P. Myers, in Chromatographia, June 2007, Vol. 65