Life Science


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Fluorescence Imaging and Biological Quantification

Raquel Seruca, Jasjit S. Suri, J. Miquel Sanches
September 11, 2017

This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It...

Histochemical and Cytochemical Methods of Visualization

Jean-Marie Exbrayat
November 16, 2016

Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity....

Optical Nanoscopy and Novel Microscopy Techniques

Peng Xi
September 19, 2014

Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades. The objective of...

Physics of Schottky Electron Sources: Theory and Optimum Operation

Merijntje Bronsgeest
July 25, 2014

The Schottky electron emitter is a predominant electron-emitting source in today’s electron beam equipment. This book comprehensively covers the Schottky emitter, dealing with its theoretical as well as practical aspects. The main questions that are addressed in this book are: what is the Schottky...

The Fluorescent Protein Revolution

Richard N. Day, Michael W. Davidson
April 28, 2014

Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a new era of investigations in cell biology, medicine, and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel...

Introduction to Fluorescence

David M. Jameson
January 22, 2014

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to...

Coherent Raman Scattering Microscopy

Ji-Xin Cheng, Xiaoliang Sunney Xie
October 29, 2012

The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions...

Linear and Chiral Dichroism in the Electron Microscope

Peter Schattschneider
March 01, 2012

This book describes energy loss magnetic chiral dichroism (EMCD), a phenomenon in energy loss spectroscopy discovered in 2006. EMCD is the equivalent of XMCD but is based on fast probe electrons in the electron microscope. A spatial resolution of 2 nm has been demonstrated, and the lattice-resolved...

Dynamic Force Spectroscopy and Biomolecular Recognition

Anna Rita Bizzarri, Salvatore Cannistraro
January 25, 2012

Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming...

Introduction to Holography

Vincent Toal
September 28, 2011

Over the course of its 60-year history, holography has enabled new insights into the nature of light and has contributed to innovative applications, including many unrelated to optics. Introduction to Holography explains how to use holographic techniques to solve specific problems in a variety of...

Life at the Nanoscale: Atomic Force Microscopy of Live Cells

Yves Dufrene
August 29, 2011

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of...

An Introduction to Microscopy

Suzanne Bell, Keith Morris
October 21, 2009

Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many...