1st Edition
Revival: Biofilms (1995) Methods for Enzymatic Release of Microorganisms
This text presents a new technique for detecting microorganisms, specifically bacteria found in all levels of the biosphere. It also discusses methods for enzymatic release of these microbes as well as their interactions in all ecosystems. Drawings and micrographs help to illustrate this concept.
Part one is devoted to the mechanisms of adherence. The second part discusses microbial ecology and the bacterial population of tissues as well as both land and aquatic microbiocenoses in general. Part three specifically covers technique. What is known about the mechanisms of adherence justifies the choice of techniques suggested. Applications in areas such as nature, medicine, environmental hygiene and the food industry are discussed.
The explanation of useful techniques, the author's research results, and practical application methods make this volume an essential reference tool for researchers, technicians and practitioners.
Structures and Mechanisms
Definition
Review of Membrane Structures
Membrane Proteins
Renewal of Membrane Constituents
Adherence Mechanisms
Adsorption-Ionic Zone
The Roles of Polycations
Adhesion-Second Reversible Stage
Irreversible Adherence-Adhesins
Glycocalyx
Fibrillae
Other Possible Adhesins
Spores
A Few Genetic Reminders
Chemical Elements of Adherence
The Bacterial Wall
Polysaccharides
Biosynthesis of Polysaccharides
Main Bacterial Polysaccharides
Lipopolysaccharides
Comment
Proteins
Polypeptides and Derivates
Lipoproteins and Derivative Complexes
Particularities of Mycobacteria
Composition Differences of the Bacterial Walls
Part II: Bacteria Life in the Wild
Adherence in Living Tissues
Cellular Receptors
Adherence to a Few Organs and Organisms
Man and Animals
Microbisms of the Mouth and Teeth
Microbisms of the Digestive Tube
Microbisms of the Stomach
Microbisms of the Urogenital Apparatus
Natural Microbiocenoses
Vegetals
The Rhizosphere
Soils and Sediments
Aquatic Environments
Gas/Liquid Interfaces
Nonmiscible Liquid Interfaces
Microbial Activity on Interfaces
Role of Plankton in Aquatic Environments
Polymorphism of Bacteria in Nature, Agglomerates
Inert Surfaces
Part III: Enzymatic Release Techniques of Bacteria
Basic Techniques
Introduction
Attack of Host Sites or Substrates
Inorganic or Inert Surfaces
Organic Surfaces
Analysis of North Atlantic Seawater
Useable Enzymes
Optimal Conditions of Useable Enzymes
Practical Applications
Techniques and Methods
Quantitation of the Enzymes
Conservation of the Enzyme Solutions
Standard Method
Specific Method
Stages of Release
Experiments With Only Enzymes
On Marine Sediment
Experimentation on Mussel Grindings
Treatment of Infected Urine
Experiment on Inert Powders
Association of Enzymes-Antibiotics
Experiment Using Bacteria Marked With Radioelements
Special Case of Fat
Experiment by Richelle Maurer
Experiment by E. Ebiou
Experiment by Patrick Brisou
Postface
Bibliography
Index
Biography
Jean F. Brisou is a national corresponding member of the Academy of Medicine.
