1st Edition

Separation Processes in Biotechnology

By Juan A. Asenjo Copyright 1990

    Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations

    Series Introduction

    Foreword (Alan S. Michaels)

    Preface

    I Separation Processes

    Selection of Operations in Separation Processes

    Juan A. Asenjo

    Chemical, Physical, and Biochemical Concepts in Isolation and Purification of Proteins

    Milton T. W. Hearn and Birger Anspach

    II Product Release and Recovery

    Protein Secretion Systems in Microbial and Mammalian Cells

    Donald T. Moir and Jen-I Mao

    Cell Disruption by Homogenizer

    Cady R. Engler

    Bead Mill Disruption

    Horst Schutte and Maria-Regina Kula

    Enzymatic Cell Lysis for Product Release

    Juan A. Asenjo and Barbara A. Andrews

    Chemical Permeabilization of Cells for Intracellular Product Relase

    Thomas J. Naglak, David J. Hettwer, and Henry Y. Wang

    III Concentration and Separation Operations

    Membrane Systems

    A. G. Fane and J. M. Radovich

    Ion-Exchange Processes

    Charles D. Shuey

    Aqueous Two-Phase Separations

    Per-Ake Albertsson, Gote Johansson, and Folke Tjerneld

    Precipitation

    Charles E. Glatz

    IV Purification Operations

    Ion Exchange in Purificatons

    Nien-Hwa Linda Wang

    Process Affinity Chromatography

    Yannis D. Clonis

    Reversed-Phase and Hydrophobic Interaction Chromatography of Peptides and Proteins

    Ziad El Rassi, Ann L. Lee, and Csaba Horvath

    Analysis of Effluent Profiles in Large-Scale Liquid Chromatography

    J. Hong

    Electrically Driven Separation Processes: Analytical and Preparative Methods

    Cornelius F. Ivory

    V Design of Separation Processes

    Synthesis of Downstream Processes

    George J. Prokopakis and Juan A. Asenjo

    Automation in Protein Purification: The Use of Expert Systems for Control of Process Chromatography

    Andrew C. Kenney

    Downstream Processing Plant and Equipment

    M. P. Brocklebank

    Downstream Process Economics

    Rajiv Datar and Carl-Gustaf Rosen

    Index

    Biography

    Juan A. Asenjo is a Reader in Biochemical Engineering at the University of Reading, England. He was previously Associate Professor of Chemical and Biochemical Engineering at Columbia University, New York, New York. The author or coauthor of numerous publications, he is a member of the American Chemical Society, American Institute of Chemical Engineers, New York Academy of Sciences, and the Permanent Committee of Biotechnology of the Interciencia Association of the American Association for the Advancement of Science. Dr. Asenjo received the Ing. Civ. Quim degree (1974) in chemical engineering from the University of Chile, M.Sc. degree (1975) in food engineering from the University of Leeds, England, and Ph.D. degree (1978) in biochemical engineering from the University College, London, England.