Dr. Sunit K. Singh, Ph.D
Professor of Molecular Immunology
Molecular Biology Unit, Institute of Medical Sciences (IMS),
Banaras Hindu University (BHU), Varanasi-221005, U.P, India
E-mail: [email protected]
URL: http://www.ccmb.res.in/scientistdetails.php?grpid=29

Research Interests: Neurovirology and Inflammation Biology, Host-pathogen interaction in Hemorrhagic Fever Virus infections

Address of correspondence:
Molecular Biology Unit, Institute of Medical Sciences (IMS),
Banaras Hindu University (BHU), Varanasi-221005, U.P, India

Personal data:
Citizenship: Indian
Marital status: Married
Residence: A-2, New Medical Enclave,BHU Campus, Banaras Hindu University (BHU), Varanasi-221005, U.P, India.

Education
BFSc  GB Pant University of Agriculture & Technology, Pantnagar, India.
MFSc  CIFE(Deemed University-ICAR), Mumbai, India.
Ph.D  Institute of Molecular Infection Biology, Univ. of Wuerzburg, Wuerzburg, Germany.

Professional Experience:
1. 19-03-17-till date Professor (Molecular Immunology), Molecular Biology Unit, Institute of Medical Sciences (IMS), Banaras Hindu University (BHU), Varanasi-221005, U.P, India
2.  19-03-14-18-03-17, Associate Professor (Molecular Immunology), Molecular Biology Unit, Institute of Medical Sciences (IMS), Banaras Hindu University (BHU), Varanasi-221005, U.P, India
2. 19-12-2006-18-03-14 Scientist, Section of Infectious Diseases, Centre for Cellular and Molecular Biology CCMB), Hyderabad, India.
3. 01/05/2013-30/05/2013 Visiting Scientist, Department of Pathology & Immunology, University Medical Centre, University of Geneva, Geneva, Switzerland.
4. 01/05/2012-31/05/2012 Visiting Scientist, Department of Microbiology, College of Veterinary Medicine, Chonbuk National University, Republic of Korea.
5. 01/05/2010-15/06/2010 Visiting Scientist, Section of Arbovirology, Dept of Parasitology, Academy of Science Czech Republic, Czech Republic.
6. 10/10/2009- 31/10/2009 Visiting Scientist, Dept of Pediatric Infectious Diseases and Immunology, Uni-Kinderklinik, University of Wuerzburg, Wuerzburg, Germany.
7. 23/03/2008- 22/09-2008 Visiting Scientist, Dept of Pathology, Dept of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York (USA).
7. 07/2005 – 11/2006 Postdoctoral Scholar, Department of Neurology, University of California Davis Medical Centre, Sacramento, California (USA).
8. 01/2005 – 06/2005  Postdoctoral Associate, (Advance training Scheme on Lyme borreliosis), Section of Rheumatology, Dept of Internal medicine, Yale University, School of Medicine, New Haven, Connecticut, USA.
9. 11/2001 – 12/2004, Research Fellow (Biologist), Department of Paediatric Rheumatology, Osteology, and Infectious Diseases, Children's Hospital, University of Wuerzburg, Germany.
10. 25/05/2000 – 31/10/2001, Senior Research Fellow, National Bureau of Fish Genetic Resources (NBFGR), Lucknow, (U.P) India.

Presentations in Seminar and Symposia:
1. Singh S. K, Wirsing A, Bar, V, Morbach, H, Frosch, M, Girschick, H. J, 2002. Regulation der Chemokin- Genexpression in humanen Synovialzellen durch verschiedene Borrelia burgdorferi- isolate. (Abstract in German). Akt. Rheumatol (2002) 27, S8.
2. Morbach. H, Samfass, U, Singh, S. K., Lipsky, P. E, Girschick, H. J, 2003, Der Einfluss von cyclophosphamid auf die differenzielle Genexpression der Gene RAG-1 und RAG-2 in CD5+ und CD5- IgD+ peripheren B-Zellen bei kindlichem SLE (Abstract in German). 13th conference of the working group on Juvenile and adolescent arthritis. Aktuelle Rheumatologie (2003), 28:285.
3. Holl, A, Suerbaum, S, Singh, S. K, Wirsing, A, Girschick, H. J, 2002 Seronegative Lyme Borreliose als Differenzialdiagnose einer frühkindlichen Oligoarthritis. (Abstract in German) In Annual meeting of the German collaborative group on Juvenile Arthritis: Hospital for Pediatric Rheumatology Garmisch- Partenkirchrn, Germany, Akt. Rheumatol (2002) 27, S14.
4. S. K. Singh, A. Wirsing, V. Baar, H. Morbach, M. Frosch and H. J. Girschick, 2003, Expression of chemokines, metalloproteinases and cyclooxygenases in human synoviocytes by different Borrelia burgdorferi isolates. (Abstract). Clin Exp Rheumatology (2003) Vol 21/4, p546.
5. H. Morbach, U. Samfass, S. K. Singh, P. E. Lipsky, H. J. Girschick, 2003, Expression of RAG1, RAG 2 and VpreB genes in IgD+CD5+/- Peripheral B cells during Cyclophosphamide treatment in Pediatric SLE (Abstract). Clin Exp Rheumatology (2003) Vol 21/4, p546.
6. S. K. Singh, A. Wirsing, V. Baar, H. Morbach, M. Frosch and H. J. Girschick, 2003, Expression of Chemokines, Metalloproteinases and Cyclooxygenases in Human Synoviocytes by different Borrelia burgdorferi Isolates (Abstract). Arthritis and Rheumatism (2003) 48 Vol 9, S519.
7. S. K. Singh, A. Wirsing, V. Baar, H. Morbach, M. Frosch and H. J. Girschick, 2003, Expression of Chemokines, Metalloproteinases and Cyclooxygenases in Human Synoviocytes by different Borrelia burgdorferi Isolates (Abstract). 13th conference of the working group on juvenile and adolescent arthritis. Aktuelle Rheumatologie (2003), 28:286.
8. H. Morbach, U. Samfass, S. K. Singh, P. E. Lipsky, H. J. Girschick, 2003, Expression of RAG1, RAG 2 and VpreB genes in IgD+CD5+/- Peripheral B cells during Cyclophosphamide treatment in Paediatric SLE (Abstract). Arthritis and Rheumatism (2003) 48 Vol 9, S192.
9. H. J. Girschick, H. Morbach, U. Samfass, S. K. Singh, P. E. Lipsky, H. J. Girschick, 2003 Expression of RAG-1 and RAG-2 genes in peripheral B cells of children and adults with SLE, 39th annual convention for paediatric research. Eur J Pediatrics (2003) 162: R6.
10. H. J. Girschick, H. Morbach, S. K. Singh, Chemokine expression von synovialzellen nach infektion mit Borrelia burgdorferi. Monatsschrift f. kiderheilkunde (2003) 151/ Suppl.1.
11. H. Morbach, S.K. Singh, C. Faber, A. Grammer, P.E. Lipsky, H.J. Girschick, RAG1 expression by peripheral blood B cells of pediatric patients with SLE, 14th Annual conference of working groups on Juvenile and Adolescent arthritis,Germany, Aktuelle Rheumatologie (2004), 63:264.
12. S.K Singh, H. Morbach, T. Nanki, C. Faber, V. Bar,  H.J.Girschick, Differential expression of matrix metalloproteinases and cyclooxygenase in synovial cells infected by Borrelia burgdorferi. 5th EULAR Congress, Berlin, 2004.
13. H. Morbach, S.K. Singh, C. Faber, A. Grammer, P.E. Lipsky, H.J. Girschick, RAG1 expression by peripheral blood B cells of pediatric patients with SLE. 5th EULAR Congress, Berlin, 2004.
14. S.K Singh, H. Morbach, C. Faber, H.J.Girschick, Expression of Recombination activating genes in human B cells after exposure to Borrelia burgdorferi, EULAR, Vienna, 07/05, 2005.
15. S.K Singh, H. Morbach, C. Faber, H.J.Girschick, Expression of Recombination activating genes in human B cells after exposure to Borrelia burgdorferi PRES annual Congress. Paris 09/05, Clin Exp Rheumatol 2005, 23: S-8.
16. S.K Singh, H. Morbach, C. Faber, H.J.Girschick, Expression of Rekombination aktivating genes in human B cells after exposure to Borrelia burgdorferi, (Abstract in German) DGPI, Düsseldorf 06/2005.
17. Sunit Kumar Singh, Maryam Urooj, Salini Krishnan, Ritu Mishra, Chintan Chhatbar, Makoto Horiuchi, David Pleasure, 2009, MicroRNA profiling of Rat brain Oligodendroglial lineage Cells, “BIOMICS Hands-on Workshop & Conference” organized by Weizmann Institute of Science, Israel.
18. Manish K. Johri, Ritu Mishra, Chintan Chhatbar, Salini Krishnan, Sunit K. Singh, (Mar. 6-10, 2010), Role of HIV Tat Protein in HIV Pathogenesis, “Second Advanced Summer School in Africa on “Molecular mechanism of Viral infection and Propagation” organized by IUBMB/ICGEB/FEBS/FASBMB/UNESCO, Hermanus, South Africa.
19. Ritu Mishra, Chintan Chhatbar, Sunit K. Singh, (Oct. 17-19, 2012), HIV-1 Tat C mediated regulation of TRAF3 by microRNA 32 in human microglia” in XXXVI All India Cell Biology Conference and International Symposium organized by Bhabha Atomic Research Centre, Mumbai, India
Meeting Report:
         Sumit G. Gandhi, Sunit K.Singh, Bharat B. Chattoo, 2010, Integrating the Omics, Current Science 98:6, 742-744.
Book Chapters:
1. Bhadra, U, Singh, S.K., Hajeri, P.B., Bhadra, M. (2009) microRNA tales in fly development”, Regulation of gene expression by small RNAs. In: R.K.Gaur and J.J.Rossi (ed.) Regulation of Gene Expression by small RNAs (ISBN: 978-0-8493-9169-9. Taylor & Francis Group, CRC Press. Pp.123-147.
2. Singh, S.K., Hajeri, P.B. (2012) RNAi: From Basics to Therapeutics. In: D. Whitehouse and R. Rapley (ed.) Molecular and Cellular Therapeutics (ISBN: 978-0-470-74814-5). John Willey & Sons Publication, Willey-Blackwell Press. Pp.123-147.
3. Mishra, R, Singh, S.K, (2013) Human Immunodeficiency Virus (HIV) Neuropathogenesis, Sunit K. Singh & Daniel Ruzek (ed.), 2013, titled “Neuroviral Infections: RNA Viruses and Retroviruses”, ISBN 1466567201, 9781466567207, Publisher: Taylor & Francis/CRC Press, USA.
4. Lahoti, J.A, Mishra, R, Singh, S.K (2013) Vascular Endothelial Dysfunctions: Viral Attack and Immunological Defense, Sunit K. Singh & Daniel Ruzek (ed.), 2013, titled “Viral Hemorrhagic Fevers”, ISBN-10:1439884293| ISBN-13: 978-1439884294 Publisher: Taylor & Francis/CRC Press, USA, (in Press).
Published Books:
1. Edited by Sunit K. Singh & Daniel Ruzek, 2013, titled “ Neuroviral Infections: General Principles and DNA Viruses”, ISBN-10: 1466567198, ISBN-13: 978-1466567191 Publisher: Taylor & Francis/CRC Press, USA.
2. Edited by Sunit K. Singh & Daniel Ruzek, 2013, titled “Neuroviral Infections: RNA Viruses and Retroviruses”, ISBN 1466567201, 9781466567207, Publisher: Taylor & Francis/CRC Press, USA.
3. Edited by Sunit K. Singh & Daniel Ruzek, 2013, titled “Viral Hemorrhagic Fevers”, ISBN-10:1439884293| ISBN-13: 978-1439884294 Publisher: Taylor & Francis/CRC Press, USA, (in Press).

Selected Publications:
1. S. K. Singh and H.J.Girschick, 2003, Tick-host interaction and their immunological implications in Tick borne diseases, Current Science 85:9, 1284-1298.
2. S. K. Singh and H. J. Girschick, 2004, Lyme borreliosis: from infection to autoimmunity, Clinical Microbiology and Infection, 10(7): 598-614. (Impact Factor: 4.540)
3. S. K. Singh and H. J. Girschick, 2004, Molecular survival strategies of the Lyme disease spirochete Borrelia burgdorferi; Lancet Infectious diseases, 4(9): 575-583. (Impact Factor: 16.16)
4. S. K. Singh, H. Morbach, T. Nanki, C. Faber, V. Baar, and H. J. Girschick, 2004, Differential expression of Matrix metalloproteinases and Cyclooxygenases in synovial cells exposed to different Borrelia burgdorferi isolates Geho and B31, Inflammation Research, 53(12) 689-696. . (Impact Factor: 2.109)
5. S. K. Singh, H. Morbach, T. Nanki, A. Wirsing and H. J. Girschick, 2005, Differential expression of Chemokines in Synovial cells exposed to different Borrelia burgdorferi isolates, Clinical and Experimental Rheumatology ,23(3): 311-22. (Impact Factor: 2.396)
6. H. Morbach, S. K. Singh, C. Faber, A. Grammer, P. E. Lipsky, H. J. Girschick, 2005, Analysis of RAG expression by peripheral blood CD5+ and CD5- B cells of patients with childhood Systemic Lupus Erythematosus. Annals of Rheumatic Diseases,65(4): 482-7. (Impact Factor: 8.727)
7. S. K. Singh, V. Baar and H. J. Girschick, 2005, Expression of ICAM-1, ICAM-2, NCAM-1 and VCAM-1 by human synovial cells exposed to Borrelia burgdorferi in vitro. Rheumatology International, 26(9): 818-827. (Impact Factor: 2.0)
8. C. Faber, H. Morbach, S.K. Singh and H.J. Girschick, 2006, Differential expression patterns of Recombination Activating Genes (RAG) in individual mature B cells in Juvenile Idiopathic Arthritis Annals of Rheumatic Diseases 65(10):1351-6. (Impact Factor: 8.727)
9. S. K. Singh and H. J. Girschick, 2006, Toll like receptors in Borrelia burgdorferi induced inflammation, Clinical Microbiology and Infection, 12(8): 705-717.
(Impact Factor: 4.540)
10. David Pleasure, Athena Soulika, Sunit K Singh, Vittorio Gallo, Peter Bannerman, 2006, Inflammation in white matter: Clinical and pathophysiological aspects Mental Retardation and Developmental Disabilities Research Reviews, 12(2): 141-146. (Impact Factor: 3.8)
11. S. K. Singh, 2007, Topical microbicides against HIV spread: What, Where and Why? (Editorial) Future Virology 2(3), 1-6. (Impact Factor: 1.21)
12. S. K. Singh, 2007, Endogenous Retroviruses-Suspects in Disease World. Future Microbiology 2(3), 269-275. (Impact Factor: 3.82)
13. S. K. Singh, 2007, MicroRNAs: From Neurogeneration to Neurodegeneration Pharmacogenomics;8(8):971-8. (Impact Factor: 3.974)
*Considered in the list of Top 10 articles” in miRNAs and Neurodegeneration domain.
14. S. K. Singh, 2007, HIV spread among women (Editorial) Expert Reviews of Antiinfective Therapy 5(5):755-758. (Impact Factor: 3.974)
15. S. K. Singh, M Bhadra, H. J. Girschick, U Bhadra 2008, MicroRNAs: Micro in size but macro in function, The FEBS Journal 275 (20), 4929-44. (Impact Factor: 3.790)
** Figure selected as the cover page of the journal.
16. S. K. Singh,2008, Oral Polio Vaccine: A matter of debate Future Microbiology 3(4):383-385. (Impact Factor: 3.82)
17. Morbach H, Richl P, Faber C, Singh SK, Girschick HJ, 2008, The kappa immunoglobulin light chain repertoire of peripheral blood B cells in patients with juvenile rheumatoid arthritis, Molecular Immunology, 45(14):3840-6. (Impact Factor: 3.023)
18. S. K. Singh, 2008, RNA interference and its therapeutic potential against HIV infection. Expert Opinion on Biological Therapy 8(4):449-461. (Impact Factor: 3.505)
19. PB Hajeri, SK Singh, 2009, siRNAs: their potential as therapeutic agents - Part I. Designing of siRNAs, Drug Discovery Today, Sep; 14(17-18):851-8. (Impact Factor: 7.155)
20. SK Singh, PB Hajeri, 2009, siRNAs: their potential as therapeutic agents - Part II. Methods of delivery, Drug Discovery Today, Sep; 14(17-18):859-65. (Impact Factor: 7.155)
21. SK Singh, RK Gaur, 2009, Progress towards therapeutic application of RNA interference for HIV infection, BioDrugs, 23 (5): 269–276. (Impact Factor: 3.443)
22. MK. Johri, R. Mishra,  C. Chhatbar, SK. Unni, SK. Singh, 2011, Tits and bits of HIV Tat Protein. Expert Opin Biol Ther. 11 (3) 269-83. (Impact Factor: 3.505)
23. SK. Unni, D. Růžek, C. Chhatbar, R. Mishra, MK. Johri, SK. Singh, 2011, Japanese encephalitis virus: From Genome to Infectome. Microbes and Infection. 13(4) 312-21. (Impact Factor: 3.005)
24. C. Chhatbar, R. Mishra, SK. Singh, 2011, HIV Vaccine: Hopes and Hurdles. Drug Discov Today.,Nov;16(21-22):948-56, (Impact Factor: 7.155)
25. SK. Singh, SK. Unni, 2011, Chikungunya Virus: Host Pathogen Interaction. Reviews in Medical Virology, 21(2)78-88. (Impact Factor: 7.200)
26. D. Růžek, J. Salát, SK. Singh, and J. Kopecký, 2011 Breakdown of the Blood-Brain Barrier During Tick-Borne Encephalitis in Mice Is Not Dependent on CD8+ T-cells PLoS One. 2011;6(5):e20472. (Impact Factor: 4.2)
27. Y. W. Han, S. K. Singh, S. K. Eo, 2012, The Roles and Perspectives of Toll-Like Receptors and CD4+ Helper T Cell Subsets in Acute Viral Encephalitis. Immune Network. 12(2):48-57.
28. R Mishra, C Chhatbar and SK Singh, 2012, HIV-1 Tat C takes on microRNA mediated regulation of TNF receptor associated factor-3 (TRAF3) in human microglial cells, Journal of Neuroinflammation, 18;9:131 (PMID: 22709905) (Impact Factor: 5.789)
29. R Mishra, and SK Singh, 2013, HIV-1 Tat C modulates expression of miRNA-101 to suppress VE-Cadherin in Human Brain Microvascular Endothelial Cells, The Journal of Neuroscience 33(14):5992-6000; doi:10.1523/JNEUROSCI.4796-12.2013, (Impact Factor: 7.23). (Included in F1000)
F1000Prime Recommendations, Dissents and Comments for [Mishra R and Singh SK, J Neurosci 2013, 33(14):5992-6000]. In F1000 Prime, 15 May 2013; F1000Prime.com/718001871.
30. G.D. Manocha, R. Mishra, N. Sharma, K.L. Kumawat, A. Basu, S.K. Singh, 2014, Regulatory role of TRIM21 in type-I interferon pathway in Japanese encephalitis virus infected human microglial cells Journal of Neuroinflammation, Feb 1;11:24. doi: 10.1186/1742-2094-11-24.
31. R. Mishra, S.K. Singh, 2014, HIV-1 Tat C phosphorylates VE-cadherin complex and increases human brain microvascular endothelial cell permeability, BMC Neuroscience 2014 Jun 26; 15(1):80. doi: 10.1186/1471-2202-15-80).
32. S.P Selvamani, R. Mishra, S.K. Singh, 2014, Chikungunya virus exploits miR-146a to regulate NF-ҡB pathway in human synovial fibroblasts, PLoS One. Aug 1; 9(8):e103624. doi: 10.1371/journal.pone.0103624.
33. V. Jadhav; K-H. Krause; S.K. Singh, 2014, HIV-1 Tat C modulates NOX2 and NOX4 expressions through miR-17 in Human Microglial Cells, Journal of Neurochemistry. Dec; 131(6):803-15.
34. M.K. Johri, N. Sharma, S.K. Singh, 2015, HIV Tat Protein: Is Tat-C much trickier than Tat-B? Journal of Medical Virology, Aug;87(8):1334-43.
35. N. Sharma, R. Verma, K.L Kumawat, A. Basu and S.K Singh, 2015, miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells, Journal of Neuroinflammation, Feb 18;12:30. doi: 10.1186/s12974-015-0249-0
36. N. Sharma, S.K. Singh, 2016, Implications of Non-coding RNAs in Viral Infections, Reviews in Medical Virology, doi: 10.1002/rmv.1893.
37. N. Sharma, K.L. Kumawat, M. Rastogi, A. Basu, S.K. Singh, 2016, Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5, Scientific Reports 2016 Jun 10;6:27685. doi: 10.1038/srep27685
38. M. Rastogi, N. Sharma, S.K. Singh, 2016, Flavivirus NS1: A Multifaceted Enigmatic Viral protein, Virology Journal  2016 Jul 29;13(1):131.
39. S.K. Singh, 2016, Overview on the tricks of HIV Tat to hit the Blood-Brain Barrier, Current HIV Research, 2016 Oct 6. [Epub ahead of print]
40. M. Rastogi, N. Srivastava, S K. Singh, 2017, Exploitation of microRNAs by Japanese Encephalitis virus in human microglial cells, Journal of Medical Virology;1–7. https://doi.org/10.1002/jmv.24995
41. M. Agrawal, M. Rastogi, S. Dogra N. Pandey, A. Basu, S. K. Singh, 2019, Chandipura Virus changes cellular miRNome in human microglial cells Journal of Medical Virology, 2019 Apr 24. doi: 10.1002/jmv.25491.
42. M. Rastogi, S. K. Singh, 2019, Modulation of type-I Interferon response by hsa-miR-374b-5p during Japanese Encephalitis Virus infection in human microglial cells Front. Cell. Infect. Microbiol., 09 August 2019 | https://doi.org/10.3389/fcimb.2019.00291


Education
Ph.D, (Molecular Infection Biology), University of Wuerzburg, Wuerzburg, Germany
Areas of Research / Professional Expertise
Neurovirology and Inflammation Biology, Neuroimmunology, Hemorrhagic Fever Viruses
Personal Interests
To study the mechanisms of pathogenesis of Emerging viruses
Websites