This is an essential bench book that describes the molecular biology, structure, detection, purification, and pathogenesis of viroids and satellites. The volume begins with an overview of the current status of the field, followed by four chapters describing details of methodology, nucleic acid probes, purification, sequence variation, complementary RNA probes, polyacrylamide gel electrophoresis and mutational analysis of viroid movement and pathogenicity. Remaining chapters discuss such topics as viroids that cause apple scar disease in China, the cadang-cadang disease of palms in the Pacific area, viroid-like satellite RNAs, and a comparison of plant viroids with the human-pathogenic delta agent. The book will be a major reference work on viroids for years to come and an essential resource for virologists, molecular biologists, microbiologists, geneticists, biochemists, biomedical investigators, plant pathologists, and agricultural researchers.
Table of Contents
Preface. Contributors. The Frontier of Life: The Viroids and Viroid-Like Satellite RNAs (Theodor O. Diener). Comparative Structure of Viroids and Their Rapid Detection Using Radioactive and Non-Radioactive Nucleic Acid Probes (James L. McInnes and Robert H. Symons). Viroids-Purification, Characterization, Diagnosis, and Detection of Sequence Variation: Application to the Carnation Stunt Associated Viroid RNAs (Peter F. Palukaitis, Gael Kurath, and Guido Boccardo). Use of Complementary RNA as Diagnostic Probes for Viroids (Chuji Hiruki). Return-Polyacrylamide Gel Electrophoresis for the Detection of Viroids (Rudra P. Singh). Mutational Analysis of Viroid Pathogenicity and Movement (Robert A. Owens and Rosemarie W. Hammond). Pear as a Natural Host of Apple Scar Skin Viroid (Po Tien). The Cadang-Cadang Viroid Disease of Palms (Karl Maramorosch). Satellite Tobacco Ringspot Virus RNA: Self-Cleavage and Ligation Reactions in Replication (George Bruening, Paul A. Feldstein, Jamal M. Buzayan, Hans van Tol, Joanna deBaer, Geoffrey R. Gough, Peter T. Gilham, and Fritz Eckstein). The Delta Agent: A Human Pathogen with Similarities to Viroids and Circular Satellite RNAs (Andrea D. Branch, Bonnie J. Levine, and Hugh D. Robertson). Index.