The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.
Table of Contents
Introduction to PCR for Viral Diagnosis (J.P. Clewley). The Current Role of PCR in Diagnostic and Public Health Virology (D.W.G. Brown). PCR in Situ: New Technologies with Single-Cell Resolution for the Detection and Investigation of Viral Latency and Persistence (K.A. Staskus, J.E. Embretson, E.F. Retzel, J. Beneke, and A.T. Haase). PCR for the Detection and Characterization of Viral Agents of Gastroenteritis (J. Green and J.P. Norcott). PCR Detection and Analysis of Hepatitis Viruses (B.H. Robertson). Application of PCR to Influenza, Measles, and Mumps (J.S. Robertson and T. Forsey). Detection and Analysis of Human Picornaviruses (G. Stanway and T. Hyypiä). Relevance of PCR to Flavivirus Research (D.A. Gadkari). Polymerase Chain Reaction for Rubella Virus Diagnosis (W.F. Carman and E.A.B. McCruden). PCR Technology for Lyssavirus Diagnosis (N. Tordo, H. Bourhy, and D. Sacramento). PCR Methods in the Diagnosis of HIV-1 and HIV-2 Infection (P. Balfe). PCR for the Detection and Discrimination of Human T-Lymphotropic Virus Type I and Type II Infections (P. Swanson, G.W. Leckie, and H.H. Lee). Herpesvirus--PCR (E. Puchhammer-Stöckl). Detection of Human Polymaviruses by PCR (P.E. Gibson). Investigation of Human Parvovirus B19 Infection Using PCR (J.P. Clewley and B.J. Cohen).