This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.
Table of Contents
Mass spectrometry and NMR: new frontiers in nuclear magnetic resonance spectroscopy - use of the Nano.NMR probe for the analysis of microgram quantities of complex carbohydrates; analysis of anionic glycoconjugates by delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Glycolipids and the new physical methods: microscale analysis of glycosphingolipids by thin-layer chromatography blotting; high-performance liquid chromatography of gangliosides; a sensitive cell suspension enzyme-linked immunosorbent assay measuring the density of cell surface ganglioside and carbohydrate antigens. Protein 0-glycosylation: effects of 0-glycosylation on protein structure - the role and characterization of heavily 0-glycosylated mucin domains; predicting mucin-type 0-glycosylation sites; experimental approaches to studying 0-glycosylation in vivo. Labelling oligosaccharides with chromophore and fluorophores: highly sensitive pre-column derivation procedures for quantitative determination of monosaccharides, sialic acids, and amino sugar alcohols of glycoproteins by reversed phase high-performance liquid chromatography; glycan labelling with the fluorophores 2-aminobenzamide and anthranilic acid. Strategies for oligosaccharides separations and sequencing: discovery and uses of novel glucosidases: resolution of oligosaccharides in capillary electrophoresis; a comparison of oligosaccharides profiling methods. Plant glycobiology: recent developments in starch structure analysis using high-performance anion-exchange chromatography with pulsed amperometric detection; red seaweed galactants - methodology for the structural determination of corallinan, a different agaroid. (Part contents)>
". . .a highly valuable manual covering most modern approaches to study glyconjugates. Being written by a group of more than 100 worldwide-known experts, it will rightly become a handbook not only for glycobiolgists, but also for specialists in the fields of bioorganic chemistry. biocehmistry, molecular biology, and pharmacy. It will become helpful for teachers and students of universities and biotechnological institutes as well."