1st Edition

Standard Operational Procedures in Reproductive Medicine Laboratory and Clinical Practice

Edited By Botros Rizk, Markus Montag Copyright 2017
    198 Pages
    by CRC Press

    198 Pages
    by CRC Press

    Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues.

    LABORATORY PROCEDURES

    1 The documentation system
    Markus Montag and Lars Johansson
    2 Culture Media Techniques
    Lars Johansson
    3 IVF incubator handling
    Jason E Swain and Alex Lagunov
    4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC)
    Markus Montag
    5 Semen collection and sample reception
    Carin Huyser
    6 Spermiogramm, Makler chamber, Neubauer chamber, CASA
    Verena Nordhoff
    7 Sperm viability testing
    Markus Montag
    8 Sperm preparation – Swim up, density gradient, migration chamber, zeta method
    Thomas Ebner
    9 Sperm selection using hyaluronan binding
    Denny Sakkas
    10 IVF - Insemination
    Markus Montag
    11 ICSI
    Thomas Freour
    12 Oocyte in vitro maturation – Isolation of COCs and culture for maturation
    Mariabeatrice Dal Canto and Giovanni Coticchio
    13 Artificial oocyte activation
    Markus Montag
    14 Cryopreservation - Sperm TESE and MESA
    Borut Kovačič
    15 Vitrification – Oocytes, 2PN, Embryos, Blastocysts
    Ana Cobo and Aila Coello
    16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts
    Etienne Van den Abbeel
    17 Oocyte selection
    Başak Balaban
    18 Zygote selection
    Martin Greuner
    19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6
    Thorir Hardarson and Julius Hreinsson
    20 Time-lapse imaging
    Cristina Hickman
    21 Assisted hatching
    Maria De Geyter and Christian De Geyter
    22 Zona manipulation at various embryo stages: Mechanical / acidic medium
    Marius Meintjes
    23 Biopsy: polar body, blastomere, trophectoderm
    Hakan Kadir Yelke and Semra Kahraman
    24 Sampling of cells after biopsy for aCGH
    Barry Behr
    25 Embryo transfer – standard and ultrasound guided
    Bettina Toth
    26 Hygenic standards in the IVF laboratory
    Vera Baukloh
    27 Quality control of IVF laboratory supplies – Mouse Embryo Assay (MEA)
    Dean E Morbeck
    28 Troubleshooting in the IVF laboratory
    David Mortimer and Sharon T Mortimer
    29 Microscopy
    Markus Montag
    30 Fertility preservation
    Markus Montag and Jana Liebenthron

    CLINICAL PROCEDURES
    31 Ultrasound evaluation in treatment management
    Ippokratis Sarris and Geeta Nargund
    32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies
    Magued Adel Aziz Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk
    33 Infertility ultrasound evaluation: One Stop Procedure
    Spiros A. Liatsikos and Geeta Nargund
    34 Office Hysteroscopy
    Linda D. Bradley
    35 Hysteroscopic management of uterine septum
    Omar Abuzeid and Mostafa Abuzeid
    36 Hysteroscopic Lysis of Intrauterine Adhesions
    Bolonduro Oluwamuyiwa and Mostafa Abuzeid
    37 Hysteroscopic Management of Submucosal Fibroids
    Salem Joseph and Mostafa Abuzeid
    38 Saline infusion sonohysterography
    Alan Bolnick and Mostafa Abuzeid
    39 General Principles of Laparoscopy
    Rubin Raju and Mostafa Abuzeid
    40 Microlaparoscopy
    Oscar D. Almeida Jr
    41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy
    Mostafa Abuzeid and Ahmed Abdel Aziz
    42 Laparoscopic Excision of a Large Endometrioma
    Rubin Raju and Mostafa Abuzeid
    43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosis
    Omar Abuzeid and Mostafa Abuzeid
    44 Laparoscopic management of mature cystic teratoma
    Rahima Sanya and Mostafa Abuzeid
    45 Tubal Cannulation for Proximal Tubal Disease
    Botros Rizk and Josh Ekladios
    46 Robotic assisted laparoscopic myomectomy
    Hashem Lofty, Arsany Bassily, Lauren Mann, and Botros Rizk
    47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization
    Hashem Lofty, Rola Turki, and Botros Rizk
    48 Unexplained infertility
    Hassan N. Sallam and Nooman H. Sallam
    49 Male Evaluation for Infertility
    Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal
    50 Hysterosalpingography
    Islam Fahmi, Mostafa Abuzeid, and Shawky ZA Badawy
    51 Intrauterine insemination: clinical perspectives
    Willem Ombelet
    52 GnRH agonist to trigger final follicular maturation in IVF
    Nayana Talukdar and Robert F. Casper
    53 GnRH agonist Protocols
    Justin Tan, Rabea Youcef Khoudja, Michael Doley, Michael Dahan and Seang Lin Tan
    54 GnRH agonists before IVF for advanced endometriosis
    Hassan N. Sallam and Nooman H. Sallam
    55 Microdose GnRH Agonist Flare Protocols
    Magued Adel Mikhail, Wael Salem, and Botros Rizk
    56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization
    Michael Dahan, Justin Tan, Baris Ata, Rabea Khoudja, Seang Lin Tan, and Botros Rizk
    57 Minimal stimulation for IVF
    Ippokratis Sarris and Geeta Nargund
    58 Ovarian Hyperstimulation Syndrome
    Magued Adel Mikhail, Daniel Antonious, Candice P. Holliday, and Botros Rizk
    59 The Prediction of OHSS
    Jan Gerris and Jana Claeys
    60 Tips on technique of embryo transfer
    Omar Abuzeid and Mostafa Abuzeid
    61 Difficult Embryo Transfers
    Gautam N. Allahbadia and Goral N. Gandhi
    62 In vitro maturation of oocytes for fertility treatment and fertility preservation
    Michael H. Dahan, Justin Tan, Rabea Youcef Khoudja, Andrew Mok, and Seang Lin Tan
    63 Complications and outcomes of IVF
    Jan Gerris
    64 Obstetric complications after in-vitro fertilization
    Brian Brocato and David Lewis
    65 Twins pregnancy after ART
    David F. Lewis and Brian Brocato
    66 IVF and endometriosis
    Graciela Kohls Ilgner and Juan Antonio Garcia Velasco
    67 Leiomyoma of the Uterus
    Shawky ZA Badawy
    68 Office Management of Polycystic Ovarian Syndrome
    Shawky ZA Badawy
    69 Adenomyosis: Imaging and Treatment
    Magued Adel Mikhail, Candice P. Holliday, and Botros Rizk
    70 Ectopic Pregnancy: Evaluation and Management
    James P. Schwayder

    Biography

    Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA

    Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany