Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins.
Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research.
Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems.
Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions.
Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.
Table of Contents
PART I SAMPLE PREPARATION
Applications of Pressure Cycling Technology (PCT) in 2DGE; F. Tao, J. Behnke, C. Li, C. Saravis, R.T. Schumacher, and N.P. Lawrence
Applications of Ion-Exchange Chromatography (IEX) to Reduce Sample Complexity Prior to Two-Dimensional Gel Electrophoresis (2DGE); M.G. Pluskal, E. Golenko, and M.F. Lopez
Use of Camelid Antibody Fragments in the Depletion and Enrichment of Human Plasma Proteins for Proteomics Applications; B. Dawson, P. Hermans, and M. ten Haaft
High-Throughput Plasma Depletion with Chicken Antibodies for Proteomic Analysis; S.W. Tam, L. Huang, D. Hinerfeld, D. Innamorati, X. Fang, W.W. Zhang, J. Pirro, and J.S. Feitelson
Immunoaffi nity Depletion of High-Abundant Proteins for Proteomic Sample Preparation; N. Zolotarjova, B.Boyes, J. Martosella, L.S. Yang, G. Nicol, K. Zhang, C. Szafranski, and J. Bailey
Isolation of Plasma Membrane Proteins for Proteomic Analysis; C. Fenselau and A. Rahbar
New Ultrafiltration and Solid Phase Extraction Techniques Improve Serum Peptide Detection; E. Chernokalskaya, S. Gutierrez, A.M. Pitt, A.V. Lazarev, and J.T. Leonard
PART II SAMPLE PREFRACTIONATION AND ANALYSES
Tools for Sample Preparation and Prefractionation in Two-Dimensional Gel Electrophoresis; A. Posch, A. Paulus, and M.G. Brubacher
Optic Nerve Fractionation for Proteomics; S.K. Bhattacharya, J. S. Crabb, S.P. Annangudi, K.A. West, X. Gu, J. Sun, V.L. Bonilha, G. Smejkal, K. Shadrach, J.G. Hollyfield, and J.W. Crabb
Fractionation of Retina for Proteomic Analyses; S.K. Bhattacharya, K.A. West, X. Gu, J.S. Crabb, K.Renganathan, Z. Wu, J. Sun, and J.W. Crabb
Reducing Protein Sample Complexity with Free Flow Electrophoresis (FFE); A. Kuchumov, G. Weber, and C. Eckerskorn
PART III APPLICATIONS OF ELECTROPHORESIS IN PROTEOMICS
Destreaking Strategies for Two-Dimensional Electrophoresis; F. Bai, S. Liu, and F.A. Witzmann
Proteomic Approaches to the Study of Rheumatoid Arthritis; M. Antonovici, K. Dasuri, H. El-Gabalawy, and J.A. Wilkins
Immunoglobulin Patterns in Health and Disease; I. Miller and M. Goldfarb
Difference Gel Electrophoresis (DIGE); M. Unlu and J. Minden
Principles and Challenges of Basic Protein Separation by Two-Dimensional Electrophoresis; A. Posch, A. Paulus, and M.G. Brubacher
Multidimensional Separation of Membrane Proteins; S. Francis-McIntyre and S.J. Gaskel
Structural Approaches to Glycoproteomics; H. Geiser, C. Silvescu, and V. Reinhold
Enrichment and Analysis of Glycoproteins in the Proteome; N.L. Wilson, N.G. Karlsson, and N.H. Packer
PART IV APPLICATIONS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Proteomic Analyses Using High-Efficiency Separations and Accurate Mass Measurements; J.M. Jacobs and R.D. Smith
Middle-Out Proteomics: Incorporating Multidimensional Protein Fractionation and Intact Protein Mass Analysis as Elements of a Proteomic Analysis Workflow; S.J. Berger, A.B. Chakraborty, K. Millea, H. Liu, and S.A. Cohen
Polymeric Monolithic Capillary Columns in Proteomics; A.R. Ivanov
PART V RELATED TECHNIQUES
Staining Proteins in Polyacrylamide Gels; G. Smejkal
Multiplexed Proteomics: Fluorescent Detection of Proteins, Glycoproteins, and Phospoproteins in Two-Dimensional (2D) Gels; B. Schulenberg
Direct Immunoprobing on Glyoxyl Agarose Composite Gels; J.R. Shainoff
Nuclear Magnetic Resonance–Driven Chemical Proteomics: The Functional and Mechanistic Complement to Proteomics; P.K. Pullela and D.S. Sem
Electrophoretic Nuclear Magnetic Resonance: Toward High-Throughput Structural Characterization of Biological Signaling Processes; Q. He and X. Song
“This volume provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may server as a guide for selection of the optimal separation strategies to solve particular biological problems. … contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery and drug discovery.”
— In Anticancer Research, Vol. 26, 2006