Discusses use of different genera of poxviruses as vectors (ortho, avi, and para)Examines factors that must be considered when using vaccinia as vectors/vaccines (e.g., attenuation, factors influencing antigenicity)Provides alternative methods of constructing recombinants and their relative benefitsIncludes comprehensive review of largest field trial undertaken with recombinant poxvirus vaccine, including the framework within which future recombinants will be assessedPresents discussions regarding the historical use of vaccinia in the eradication of smallpox
Recombinant Poxviruses provides a comprehensive examination of poxviruses with an emphasis on the potential of these viruses as new vaccines. The book considers a wide range of issues involved in producing new genetically engineered live vaccines, such as efficacy, safety, stability, cost, host range, immune response, immunization route, use of multivalent vaccines, and need for revaccination. The opening chapter describes the origin of vaccinia virus, its use to eradicate smallpox, and the pathogenesis of poxvirus infections. Subsequent chapters examine the molecular biology of poxviruses, methods of constructing vaccinia virus recombinants, and applications; the use and immune responses induced by poxvirus recombinants as live vaccines; and the important issues of the safety and immunogenicity of vaccinia virus. The book's final two chapters report the progress that has been made developing avipoxviruses and parapoxviruses as candidate recombinant vaccines.
Recombinant Poxviruses will be a welcome addition to the bookshelves of virologists, microbiologists, infectious disease specialists, and veterinarians.
Table of Contents
Vaccinia Virus as a Vaccine and Poxvirus Pathogenesis (F. Fenner). Molecular Biology of Poxviruses (B. Moss). The Design, Construction, and Use of Vaccinia Virus Recombinants (M. Mackett and G.L. Smith). Poxvirus Recombinants as Live Vaccines (W.I. Cox, J. Tartaglia, and E. Paoletti). Development and Deliberate Release of a Vaccinia-Rabies Recombinant Virus for the Oral Vaccination of Foxes Against Rabies (P.-P. Pastoret, B. Brochier, J. Blancou, M. Artois, M. Aubert, M.-P. Kieny, J.-P. Lecocq, B. Languet, G. Chappuis, and P. Desmettre). Immunogenicity and Antigen Presentation (M.E. Andrew, B.E.H. Coupar, and D.B. Boyle). Safety and Attenuation of Vaccinia Virus (R.M.L. Buller and G.J. Palumbo). Avipoxvirus Vectors (M.E.G. Boursnell). Parapoxviruses: Their Biology and Potential as Recombinant Vaccines (A.J. Robinson and D.J. Lyttle).