With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Biography
Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK
The goal is to provide both theoretical and practical information for those wishing to use real time PCR as a laboratory tool. This is a generally worthwhile aim and, with some caveats, suitably met. This is a useful book in that limitations and uses are both covered. Doody's Notes, February 2007
