2nd Edition
Plant Tissue Culture Concepts and Laboratory Exercises
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research.
The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns."
It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
PART I-INTRODUCTION
Introduction of plant tissue and cell culture
PART II-HISTORY OF PLANT TISSUE CULTURE
History of plant tissue and cell culture
PART III-SUPPORTING METHODOLOGIES
Getting started with tissue culture-Media preparation, sterile technique and laboratory equipment
Nutrition of callus cultures
Histological techniques
Microscopy and photography
Statistical analysis of plant tissue culture data
PART IV-PROPAGATION TECHNIQUES
Propagation from preexisting meristems
Micropropagation of Syngonium by shoot culture
Micropropagation of Dieffenbachia
Micropropagation of potato by node culture and microtuber production
Micropropagation of lilac
Micropropagation and in vitro flowering of rose
Propagation from nonmeristematic tissues- organogenesis
Direct shoot organogenesis from leaf explants of chrysanthemum
Shoot organogenesis of watermelon
Direct and indirect shoot organogenesis from leaves of Torenia fournieri
Shoot organogenesis from petunia leaves
Propagation from nonmeristematic tissues- Nonzygotic embryogenesis
Embryogenic callus and suspension cultures from leaves of orchardgrass
Somatic embryogenesis from seeds of melon
Somatic embryogenesis from mature peanut seed
Direct somatic embryogenesis from leaves and flower receptacles of cineraria
Somatic embryogenesis from immature seeds of yellow poplar
Introduction of somatic embryogenesis in conifers
PART V-CROP IMPROVEMENT TECHNIQUES
Use of protoplasts for plant improvement
Isolation, culture and fusion of tobacco and potato protoplasts
Isolation of protoplasts from leaves of chrysanthemum and orchardgrass
Haploid cultures
Production of haploid tobacco plants using another culture
Transformation
Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the ß-glucuronidase (GUS) reporter gene
Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens
Construction and use of a simple gene gun for particle bombardment
Germplasm preservation
Vitrification as a method to cryopreserve shoot tips
Secondary product expression in vitro
Pigment production in Ajuga cell culture
In vitro plant pathology
PART VI-SPECIAL TOPICS
Variation in culture
Tissue culture of ferns
Commercial laboratory production
Indexing for plant pathogens
Culture indexing for bacterial and fungal contaminants
PART VII-APPENDIX I: LIST OF SUPPLIERS
PART VIII-INDEX
Biography
Robert N. Trigiano
"Instructors and students alike will be delighted by the rich array of responsive plant species and the depth of choice among laboratory exercises that are available in Plant Tissue Culture Concepts and Laboratory Exercises, 2nd edn, edited by Robert N. Trigiano and Dennis J. Gray… The book is refreshingly readable, and the sequence of laboratory exercises leads the reader/student to develop increasing skills and knowledge of the subject matter… The range of subject matter included in the book is extensive… The written protocols do provide timely reminders regarding the benefits of careful labeling of explants and the identities of species/varieties… Laboratory protocols are very complete …Certainly this book will be of practical value in planning and teaching a successful course in plant tissue culture, but it also provides a wealth of general information and will serve as a useful reference for many protocols needed in the daily operations of tissue culture research laboratories. It will be a welcome addition to the library of everyone interested in the subject…"
- John Hubstenberger, New Mexico State University, in In Vitro Cellular Developmental Biology, Nov.- Dec. 2000
"The appeal of this practical manual is that it contains very detailed information on how to establish a tissue culture laboratory, from provision of lists of equipment and chemicals and their suppliers, to procedures and protocols for preparing media and carrying out experiments…I would recommend this book highly for teachers and researchers in plant tissue culture as a valuable resource for practical plant tissue culture."
-Plant Cell, Tissue and Organ Culture, Vol. 65, 173-174, 2001
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