3rd Edition

Plant Cytogenetics

By Ram J. Singh Copyright 2017
    548 Pages 143 B/W Illustrations
    by CRC Press

    548 Pages 143 B/W Illustrations
    by CRC Press

    Cytogenetics plays an important role in understanding the chromosomal and genetic architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its predecessors presenting theoretical and practical aspects of plant cytogenetics. Chapters describe correct handling of plant chromosomes, methods in plant cytogenetics, cell division, reproduction methods, chromosome nomenclature, karyotype analysis, chromosomal aberrations, genome analysis, transgenic crops, and cytogenetics in plant breeding.



    This new edition begins with a brief introduction on the historical aspect of cytogenetics and flows directly into handling of plant chromosomes by classical and modern cytological techniques, classical Mendelian Genetics, brief description of cell division, and chromosome identification by karyotype analysis. The comprehension of cytogenetics is incomplete without information on the role of aneuploidy in associating a gene on a particular chromosome, and the book covers these methodologies as a primary topic. Covering classical to modern cytogenetics, the book presents to the reader the crucial role of cytogenetics in improving crops.

    INTRODUCTION

    THE HANDLING OF PLANT CHROMOSOMES
    Introduction
    Squash Technique - Mitotic and Meiotic Chromosomes
    Smear Technique for Plant Chromosomes
    Pollen Staining
    Pollen-Stigma Incompatibility
    Fluorescence in situ Hybridization (FISH)
    Total DNA Extraction-Plant Genomic DNA
    Determination of Nuclear DNA Content of Plants by Flow Cytometry
    Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry
    Production of Wide Hybrids Through In Vitro Technique

    CELL DIVISION
    Introduction
    Mitosis
    Meiosis

    GENETIC CONTROL OF MEIOSIS
    Introduction
    Synaptic Mutants
    Genetic Control of Recombination
    Genes Responsible for Chromosome Disjunction
    Other Meiotic Mutants
    Role of Heterochromatin in Chromosome Pairing
    Diploid-like Meiosis in Allopolyploids
    Haploidy
    Male Sterility

    MODE OF REPRODUCTION IN PLANTS
    Introduction
    Sexual Reproduction
    Asexual Reproduction
    The Chromosomal Basis of Sex Determination in Plants

    KARYOTYPE ANALYSIS
    Introduction
    Nomenclature of Chromosomes
    Karyotype Analysis by Mitotic Metaphase Chromosomes
    Karyotype Analysis by Pachytene Chromosomes
    Karyotype Analysis by Flow Cytometry
    Karyotype Analysis by Image Analysis
    Chromosome Number of Economically Important Plants

    CHROMOSOMAL ABERRATIONS - STRUCTURAL CHROMOSOME CHANGES
    Deficiencies
    Duplications
    Interchanges
    Inversions

    CHROMOSOMAL ABERRATIONS - NUMERICAL CHROMOSOME CHANGES
    Euploidy
    Aneuploidy: Trisomics
    Monosomics and Nullisomics
    Alien Addition Lines
    Alien Substitution Lines

    GENOME ANALYSIS
    Introduction
    Classical Taxonomy and Genome Designations
    Genome Relationships among Diploid Species
    Genomes of Glycine
    Genomes of Gossypium
    Ancestors of Allopolyploid Species
    Genome Analysis by Molecular Methods

    CHROMOSOMAL ABERRATIONS IN CELL AND TISSUE CULTURE
    Introduction
    Chromosomal Aberrations in Callus
    Mechanism of Chromosomal Aberrations in Culture
    Chromosomal Aberrations in Regenerated Plants
    Chromosomal Aberrations in Haploid Callus and Their Regenerants
    Chromosomal Aberrations in Somatic Hybrids

    TRANSGENIC CROPS
    Introduction
    Present Status of Transgenic Crops
    Production of Transgenic Crops
    Chromosome Aberrations in Transgenic Crops
    Cytological Basis of Gene Silencing

    APPENDICES

    REFERENCES

    GLOSSARY

    Biography

    Ram J. Singh