PCR Protocols in Molecular Toxicology

1st Edition

John P. Vanden Heuvel

CRC Press
Published September 24, 1997
Reference - 256 Pages - 3 B/W Illustrations
ISBN 9780849333446 - CAT# 3344
Series: Methods in Life Sciences - Toxicology Section

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Summary

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:
  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
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