This book describes analysis techniques and results of topics such as physical backgrounds, chemical backgrounds, and principal methods of topo-optical reactions used in ultrastructure research of the ECM; orientation patterns of GAGs and collagen in different tissues/cartilage, cornea, kidney basement membranes, and skin; factors involved in the formation of submicroscopically ordered matrix structure; and cell-matrix interactions, including cytoskeleton-cell-membrane-matrix relationships. A summarization of the advantages and limitations of polarization microscopy compared to electron microscopy in ultracellular research is also included. Cell biologists, histologists, pathologists, and biochemists in connective tissue research will find this book to be an invaluable reference tool.
Table of Contents
1. A Short Overview of Major Constituents 2. Physical Backgrounds of Polarization Microscopy 3. Topo-Optical Reactions Used in Polarization Microscopic Ultrastructure Research 4. Structure of Hyaline Cartilage Matrix 5. Organic Bone Matrix 6. Structure of the Corneal Stroma 7. Structure of Kidney Basement Membranes 8. Experimental Reconstruction of the Oriented Glycosaminoglycan Structure of the Extracellular Matrix 9. Factors Involved in Formation and Maintenance of Oriented Microstructure of Matrix Constituents 10. Polarization Microscopy of Amyloid 11. Correlation Between Polarization and Electron Microscopic Findings in Ultra-Structure Research of Proteoglycans 12. Methodological Appendix.