This work presents an overview of the clinical pathology of mycobacterial and nonpathogens, data on genetically based methodologies for clinical testing, straightforward protocols for molecular manipulations, and theoretical explanations of the molecular mechanisms involved. It explains the safest and most efficient methods for detecting mycobacterium avium complex, providing the means to combat this common secondary opportunistic infection in AIDS patients.
Table of Contents
Diseases resulting from mycobacterial and nocaridal pathogens; conventional and molecular methodologies applied to handling and isolation of mycobacteria cells from clinical isolates; application of paraffin slide culture technology for isolation; speciation, antibiotic sensitivity testing and DNA extraction for mycobacterium avium complex organisms - isolation and DNA extraction for MOTT mycobacterial and nocardio-form organisms; the mycobacterial and nocardial genomes and their isolation and manipulation; mycobacterial transposable elements, insertion sequences, and repetitive DNA; DNA fingerprinting - RFLP analysis; double repetitive element-polymerase chain reaction method for subtyping mycobacterium tuberculosis strains; the application of pulsed field gel electrophoresis to strain typing of mycobacteria; isolation and characterization of mycobacterial plasmid; mycobacterial shuttle vectors - homologous and heterologus gene expression; mycobacterial genetics - gene expression and recombination; nocardia in situ hybridization; RNA extraction.
". . .a comprehensive review of the present applications of molecular biology in mycobacteriology and nocardiology. Most chapters include detailed protocols for the molecular manipulations, together with theoretical explanatory notes. It is a highly suitable reference source both for clinical and research microbiologists, as well as for specialists in molecular biology. "
"Answering the need for a comprehensive study of mycobacterial and nocardial pathogens, and promising to yield treatments for opportunistic infections associated with HIV, this eminently practical reference supplies an overview of the clinical pathology of these microbes, data on genetically based methodologies for clinical testing, straightforward protocols for molecular manipulations, and sound theoretical explanations of the molecular mechanisms involved. (Anticancer Research) ...provides a valuable overview on the molecular biology of mycobacterial and nocardial pathogens in theory and practice."
---International Journal of Medical Microbiology
"...informative and well-written."
---ASM News, 2001