1st Edition

Molecular Detection of Human Parasitic Pathogens

Edited By Dongyou Liu Copyright 2013
    895 Pages 117 B/W Illustrations
    by CRC Press

    Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites.

    The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens.

    This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes:

    • A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group
    • An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols
    • A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens

    This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.

    Introductory Remarks
    Dongyou Liu

    Section I Sarcomastigophora
    Acanthamoeba
    Hélene Yera, Muriel Cornet, and Jean-Louis Bourges
    Balamuthia
    Govinda S. Visvesvara and Yvonne Qvarnstrom
    Blastocystis
    Hisao Yoshikawa
    Dientamoeba
    Damien Stark
    Entamoeba
    Damien Stark and John Ellis
    Giardia
    Yaoyu Feng and Lihua Xiao
    Leishmania
    Clotilde Marín, Silvia S. Longoni, and Manuel Sánchez-Moreno
    Naegleria
    Mineko Shibayama, Jesús Serrano-Luna, Isaac Cervantes-Sandoval, and Víctor Tsutsumi
    Pentatrichomonas
    Tania Crucitti
    Trichomonas
    Dongyou Liu, Xiangang Kong, and Changyou Xia
    Trypanosoma
    Emily R. Adams

    Section II Ciliophora and Apicomplex
    Babesia
    Dongyou Liu and Xuedong Lu
    Balantidium
    Dongyou Liu
    Cryptosporidium
    Una M. Ryan and Rongchang Yang
    Cyclospora
    Dongyou Liu
    Isospora
    Chaturong Putaporntip and Somchai Jongwutiwes
    Plasmodium
    Dongyou Liu and Yi-Wei Tang
    Sarcocystis
    L. Vangeel, K. Houf, P. Geldhof, J. Vercruysse, R. Ducatelle, and K. Chiers
    Toxoplasma
    Kjetil Asbakk and Kristin Wear Prestrud

    Section III Platyhelminthes: Cestoda
    Diphyllobothrium
    Barbara Wicht, Raffaele Peduzzi, Hélene Yera, and Jean Dupouy-Camet
    Dipylidium
    Dongyou Liu
    Echinococcus and Echinococcosis
    Akira Ito, Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Jenny Knapp, and Yuji Ishikawa
    Hymenolepis
    B.R. Mirdha and Shabeena Rehman
    Mesocestoides
    Kerry A. Padgett, Paul R. Crosbie, and Walter M. Boyce
    Spirometra
    Dongyou Liu, Jianshun Chen, and Weihuan Fang
    Taenia
    Munehiro Okamoto and Akira Ito

    Section IV Platyhelminthes: Trematoda
    Clonorchis
    Heinz Mehlhorn, Boris Müller, and Jürgen Schmidt
    Dicrocoelium
    Donato Traversa, Vincenzo Lorusso, and Domenico Otranto
    Echinostomes
    Rafael Toledo and Bernard Fried
    Fasciola
    Dongyou Liu and Xing-Quan Zhu
    Fasciolopsis
    Veena Tandon, B. Roy, and P.K. Prasad
    Gastrodiscoides
    Dongyou Liu
    Haplorchis
    Urusa Thaenkham and Jitra Waikagul
    Heterophyidae
    Ron Dzikowski and Michael G. Levy
    Metagonimus
    Jae-Ran Yu and Jong-Yil Chai
    Nanophyetus
    Dongyou Liu
    Opisthorchis
    Paiboon Sithithaworn, Thewarach Laha, and Ross H. Andrews
    Paragonimus
    Hiromu Sugiyama, Takhellambam Shantikumar Singh, and Achariya Rangsiruji
    Plagiorchis
    Sun Huh
    Schistosoma
    and Schistosomiasis
    Antonio Muro and Luís Pérez del Villar
    Trichobilharzia
    P. Horák, F.M. Schets, L. Kolárˇová, and S.V. Brant

    Section V Nematoda
    Ancylostoma
    Jaco J. Verweij
    Angiostrongylus
    Dongyou Liu
    Anisakis
    Stefano D’Amelio, Serena Cavallero, Marina Busi, Sofia Ingrosso, Elisabetta Giuffra, Sarra Farjallah, and Graça Costa
    Ascaris
    Dwight D. Bowman and Janice L. Liotta
    Baylisascaris
    Dongyou Liu
    Brugia
    Kosum Chansiri and Supatra Areekit
    Capillaria
    Dongyou Liu
    Dioctophyme
    Dongyou Liu
    Dirofilaria
    Andrea Gustinelli, Maria Letizia Fioravanti, Monica Caffara, Lorella Garagnani, and Francesco Rivasi
    Dracunculus
    Dongyou Liu
    Enterobius
    Dongyou Liu
    Gnathostoma
    Paron Dekumyoy, Tippayarat Yoonuan, and Jitra Waikagul
    Haemonchus
    Michael B. Hildreth and Aaron Harmon
    Loa
    Fousseyni S. Touré Ndouo
    Mansonella
    Ramiro Morales-Hojas
    Marshallagia
    Jesús M. Pérez and Luca Rossi
    Necator
    Jan M. Schwenkenbecher
    Oesophagostomum
    Jaco J. Verweij
    Onchocerca
    Ramiro Morales-Hojas
    Pseudoterranova and Contracaecum
    Simonetta Mattiucci, Michela Paoletti, Stephen C. Webb, and Giuseppe Nascetti
    Strongyloides
    Dongyou Liu
    Syphacia
    Sanford H. Feldman and John H. Porter
    Thelazia
    Domenico Otranto and Donato Traversa
    Toxocara
    Anna Borecka
    Trichinella
    Edoardo Pozio and Giuseppe La Rosa
    Trichostrongylus
    Tai-Soon Yong
    Trichuris
    C. Cutillas, M. de Rojas, and R. Callejón
    Wuchereria
    Dongyou Liu and Frank W. Austin

    Section VI Arthropoda: Arachnida
    Argasidae (Soft Ticks)
    Dongyou Liu
    Demodex (Hair Follicle Mite)
    Dongyou Liu
    Dermanyssus, Ornithonyssus, and Trombicula (red, fowl, and harvest mites)
    Dongyou Liu
    Ixodidae (Hard Ticks)
    Dongyou Liu and Chong Yin
    Pyroglyphidae and Glycyphagidae (House Dust and Storage Mites)
    Dongyou Liu
    Sarcoptes (Itch Mite)
    Dongyou Liu

    Section VII Arthropoda: Insecta
    Calliphoridae, Oestridae, and Sarcophagidae (Myiasis-Causing Flies)
    Dongyou Liu
    Cimex (Bedbug)
    Dongyou Liu
    Pediculus (Body and Head Lice)
    Dongyou Liu and Sacha Stelzer-Braid
    Phthirus (Crab Louse)
    Dongyou Liu
    Pulex, Xenopsylla, and Ctenocephalides (Human, Rat and Dog/Cat Fleas)
    Dongyou Liu
    Tunga (Jigger Flea)
    Dongyou Liu

    Section VIII Arthropoda: Pentastomida
    Armillifer, Linguatula, and Porocephalus (Tongue Worms)
    Dongyou Liu

    Biography

    Dongyou Liu