Going beyond step-by-step descriptions of how to perform techniques, Methods in Neuroendocrinology examines the questions that are being asked in the field, what problems need to be overcome, and what the limits of interpretation are involved in each approach. The experimental approaches described in this book merge endocrine and neuroscientific research and offer insight into the many disciplines involved.
Demonstrating what can be learned from applying different approaches to neuroendocrine research, Methods in Neuroendocrinology leads the reader from elementary techniques to more complex methods for examining neuroendocrine function. The research methodology outlined addresses four main questions:
(1) What are the cellular and molecular mechanisms responsible for the synthesis and secretion of hormones?
(2) How do other cells influence neuroendocrine control mechanisms?
(3) How do hormones feed back on their neuroendocrine regulatory neurons?
(4) How can we use hormones as indicators of specific changes in neuroendocrine control sites in the brain?
Much more than a "cookbook", this is a resource for every researcher who wants to pursue new areas of inquiry in the science of neuroendocrinology.
Table of Contents
Introduction, L.D. Van de Kar
Studying Morphology of the Immortalized Hypothalamic LHRH Neurons, W.C. Wetsel, I. Merchenthaler, and Z. Liposits
Assessment of Neuropeptide Receptor Gene Expression Using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), J.H. Urban
Identification of Biotinylated Ligands on Specific Target Cells in the Pituitary: Studies of Regulation of Binding, G.V. Childs
Steroid Hormone Receptors and the Assessment of Feedback Sensitivity, R.J. Handa, R.H. Price, Jr., and M.E. Wilson
In Vitro Approaches to Neuroendocrinology, C.D. Sladek
Intracellular Recording from Hypothalamic Cells that Regulate Neuroendocrine Function, B.N. Smith and F.E. Dudek
Application of In Vivo Electrophysiological Approaches to the Study of Neuroendocrine Systems, J.M. Lakoski and J.E. Smith
Antisense Oligonucleotides as Tools in Neuroendocrinology, M. Morris
Studying the Role of Biogenic Amines in Neuroendocrine Regulation in Conscious Rats, G. Bagdy
The Neuroendocrinology of Stress: Behavioral and Neurobiological Considerations, B. Bohus
Issues in Quantifying Pulsatile Hormone Release, J.D. Velhuis
Neuroendocrine Challenge Tests: What Can We Learn from Them?, P.J. Cowen
"…Louis Van de Kar and a broad spectrum of researchers in neuroendocrinology have produced a useful handbook for those new to the field as well as neuroendocrinologists searching for novel approaches to old questions."
-Judith L. Turgeon, The Physiologist, October 1999