The past few years have witnessed extraordinary advances in molecular genetic techniques and the accumulation of structural genomics information and resources in both human and model organisms. With the development of new technologies and the availability of resources like the sequence of eukaryotic genomes, problems of a previously unthinkable scope are now being routinely solved in neuroscience and many other areas of biomedical research. The results of these studies, in turn, are having, and will continue to have, profound impact on experimental approaches and designs for manipulating genes, the genome, and model organisms as a means of gaining insights into nervous system functioning and complex behavior.
Methods in Genomic Neuroscience provides newcomers and experienced researchers with a reference guide for applying powerful, state-of-the-art molecular genetic techniques to the study of neural and behavioral systems. It thereby provides a foundation by which data on gene expression and function may be used to develop new therapeutic strategies for brain diseases.
Authored by researchers in the forefront of genomic neuroscience, the book addresses state-of-the-art tools and technologies for global analysis of genes influencing the nervous system and its disorders. An emphasis is placed on massively parallel approaches for analyzing the avalanche of data that is being generated from the full genomic sequence of humans and model systems. Specific topics include human sequence variation, methods for discovering disease vulnerability genes, gene-environmental interaction, gene expression analysis using DNA microarrays, random mutagenesis, gene trap approaches for studying brain development, neural stem cells, gene targeting, and gene delivery.
Table of Contents
Floyd E. Bloom, M.D.
Gene-Environment Interaction and Complex Behavior
Marla B. Sokolowski, Ph.D. and Douglas Wahlsten, Ph.D.
Current Perspectives on the Genetic Analysis of Naturally-Occurring Neurological Mutations in Mice
Wayne N. Frankel, Ph.D.
Conditional and Inducible Gene Targeting in the Nervous System
Mark Mayford, Ph.D. and Eric Kandel, M.D.
Functional Identification of Neural Genes
Lawrence H. Pinto, Ph.D. and Joseph S. Takahashi, Ph.D.
Studying Brain Development and Wiring Using a Modified Gene Trap Approach
Kevin J. Mitchell, Ph.D., Lisa V. Goodrich, Ph.D., Philip A. Leighton, Ph.D., Xiaowei Lu, Ph.D., Kathy Pinson, Ph.D., Paul Scherz, Olivia G. Kelly, Ph.D., Joel Zupicich, Paul Wakenight, Peri Tate, Ph.D., Judy Mak, Edivinia Pangilinan, Helen Rayburn, Ph.D., Danielle Rottkamp, Joe Zhong, William C. Skarnes, Ph.D., and Marc Tessier-Lavigne, Ph.D
N-Ethyl-N-Nitrosourea (ENU) Mutagenesis: Functional Analysis of the Mouse Nervous System and Behavior
Stephen Kanes, M.D., Ph.D., Gillian R. Leach, and Maja Bucan, Ph.D.
DNA Arrays and Gene Expression Analysis in the Brain
David J. Lockhart, Ph.D. and Carrolee Barlow, M.D., Ph.D.
DNA Microarrays and Human Brain Disorders
Karoly Mirnics, M.D., David A. Lewis, M.D., and Pat Levitt, Ph.D.
Full-Length cDNA Libraries: Reagents for Functional Studies of the Nervous System
Munetomo Hida, Ph.D., Yutaka Suzuki, Ph.D., and Sumio Sugano, M.D.
Methods for Gene Delivery to Neural Tissue
Jürgen A. Hampl, M.D., Alice B. Brown, M.D., Ph.D., Nikolai G. Rainov, M.D., and Xandra O. Breakefield, Ph.D.
Neural Stem Cells and Brain Repair
Lorenz Studer, M.D. and Ron McKay, Ph.D.
Statistical Methods to Discover Susceptibility Genes for Nervous System Diseases
Jurg Ott, Ph.D.
Genetic Variation Analysis of Neuropsychiatric Traits
Michael E. Zwick, Ph.D., David J. Cutler, Ph.D., Aravinda Chakravarti, Ph.D.
"It is primarily an excellent state-of-the-art overview of mouse neurogenetics, covering advanced methods of genetic manipulation and analysis in forward and reverse genetics.... Each of these practitioners and facilitators of modern neuroscience will benefit from reading this book... I recommend reading Methods in Genomic Neuroscience in its entirety - from start to finish, or from finish to start, or in any order. That is a compliment that one can rarely offer a book of the methods type."
-Nature Neuroscience, November 2001