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This up-to-date volume describes the wide variety of available methods concerning percutaneous absorption of drugs and toxins. It discusses in detail the advantages and disadvantages of each method. This unique publication provides a clear, systematic presentation of each aspect which must be considered when designing and performing skin penetration experiments and when interpreting results. It includes specific information regarding necessary supplies and equipment, along with commercial sources for these items. This state-of-the-art book is an ideal instructional manual for investigators uninitiated in performing percutaneous absorption and metabolism studies. Those involved with investigative dermatology, dermatological research, toxicology, and pharmacology will find this reference interesting and indispensable.
Table of Contents
Choice of Membranes for In Vitro Skin Uptake Studies and General Experimental Techniques. Penetrant Characteristics Influencing Skin Absorption. Instrumentation and Methodology for In Vitro Skin Diffusion Cells. Preparation of Biological Membranes. Methodology for the Execution of In Vitro Skin Penetration Determinations. In-Vivo Methods for Determining Percutaneous Absorption and Metabolism of Xenobiotics: Indirect Methods. Physical Resources Needed for In Vivo Animal Studies. Detection Methods. Data Interpretation and Analysis in Percutaneous Absorption Studies. Specialized Techniques: Congenitally Athymic (Nude) Animal Models. Specialized Technique: The Isolated Perfused Porcine Skin Flap (IPPSF). Validity of Skin Absorption and Metabolism Studies.
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