Describes basic laboratory techniques in detail, accompanied by practical examples
Contains an outstanding selection of references for follow-up work
Includes cross-referencing of methods allowing for easy access to information
Illustrates the importance of ethical considerations and safety in laboratory research
Features introductory material for each chapter, providing a better understanding of the methods and applications
Examines potential problems and notes trouble-shooting tips
Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.
Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-
Commercially available adjuvants designed for the production of antisera in the research setting
Methods for the production, purification, and characterization of antibodies
Practical guidance to researchers needing to modify antibodies
Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
Methods for applying immunolabeling to the realm of electron microscopy (EM)
New antibody sources and new ways to use antobodies
Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.
Table of Contents
Antibodies; Matthew R. Kaser and Gary C. Howard
Antigens; Paul Algate, Jory Baldridge, and Sally Mossman
Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman
Production of Polyclonal Antibodies; Lon V. Kendall
Production of Monoclonal Antibodies; Kathleen C. F. Sheehan
Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith
Purification and Characterization of Antibodies; Joseph P. Chandler
Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu
Chemical and Proteolytic Modification of Antibodies; George P. Smith
Applications; Lee Bendickson and Marit Nilsen-Hamilton
Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele
Immunoelectron Microscopy; Sara E. Miller and David N. Howell
Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze
ELISAs; John Chen and Gary C. Howard
Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard
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