Focusing on state-of-the-art gas chromatography (GC) and high-performance liquid chromatography, this guide discusses the theories behind, and applications of, the latest developments in chromatographic techniques - assessing the strengths and limitations of each methodology.;Presenting many detection methods for the first time, Lipid Chromatographic Analysis: provides a review of conventional column and thin-layer chromatography; examines a new GC technique to detect plasmalogen phospholipids; outlines a supercritical fluid chromatography for lipids; details the first systematic description of a microanalytical procedure for cholesterol and related compounds; explains a GC analysis of lipid breakdown products for monitoring biological processes such as ageing, carcinogenesis and mutagenesis; and describes techniques that allow for the simultaneous analysis of non-polar and polar lipids.
Table of Contents
Overview and Recent Developments in Solid-Phase Extraction for Separation of Lipid Classes * Thin Layer Chromatography with Flame Ionization Detection * Capillary Ionization Detection * Capillary Gas Chromatography of Myocardial Cholesterol Oxides * Gas Liquid Chromatography of Neutral Lipids * GLC and HPLC of Neutral Glycerolipids * GLC and High-performance Liquid Chromatographic Analysis of Lipid Peroxidation Products * Quantitative Analysis of Lipids by HPLC with a Flame-Ionization Detector or an Evaporative Light-Scattering Dectector * HPLC Analysis of Lipids (Analysis of Fatty Acids and Their Derivatives by a Microcolumn HPLC System) * Chromatographic Analysis of Ether-Linked Glycerolipids, Including Platelet Activating Factor and Related Cell Mediators * Gas Chromatography - Mass Spectroscopic Detection of Plasmalogen Phospholipids in Mammalian Heart Quantitative Capillary Gas Chromatography Mass Spectroscopic Detection of Plasmalogen Phospholipids in Mammalian Heart * Quantitative Capillary Gas Chromatography - Mass Spectrometry of Lipids Using Stable Isotope Dilution Methods * Supercritical Fluid Chromatographic Analysis of Lipids.
"This excellent, practical book with its detailed methodology will be a constant source of reference to those actively engaged in lipid research. . ..It is highly recommended and it should br ab addition to all libraries of laboratories in which there is an interest in the chemical and biochemical aspects of lipids. "
". . .should be of special interest to biochemical and biomedical researchers. "
". . .comprehensive, well-written and, most important, critical. . .. "
---Trends in Analytical Chemistry