Isolation and Purification of Proteins

1st Edition

Rajni Hatti-Kaul, Bo Mattiasson

CRC Press
Published February 5, 2003
Reference - 676 Pages
ISBN 9780824707262 - CAT# DK1931
Series: Biotechnology and Bioprocessing

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Summary

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

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