Isolation and Purification of Proteins

1st Edition

Rajni Hatti-Kaul, Bo Mattiasson

CRC Press
Published June 19, 2019
Reference - 676 Pages
ISBN 9780367395490 - CAT# K449025

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Summary

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

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