Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.
Table of Contents
Insect cell culture engineering - an overview, Mattheus F.A. Goosen; an overview of the molecular biology and applications of baculoviruses, John Kuzio and Peter Faulkner; bioreactor design for insect cell cultivation - a review, Janus J. Malinowski and Andrew J. Daugulis; insect cell immobilization, Mattheus F.A. Goosen; applications of insect cell gene expression in pharmaceutical research, Darrell R. Thomsen et al; scale-up considerations and bioreactor development for animal cell cultivation, Johannes Tramper et al; serum-free media, Stefan A. Weiss et al; foreign gene expression in insect cells, Donald L. Jarvis; the future of insect cell culture engineering, Spiros N. Agathos.