The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.
Table of Contents
Probes for In Situ Hybridization Histochemistry (M.E. Lewis and F. Baldino, Jr.). Detection of Messenger RNAs by In Situ Hybridization with Nonradioactive Probes (B. Bloch). In Situ Hybridization in Cells and Tissue Sections: A Study of Myelin Gene Expression during CNS Myelination and Remyelination (C.A. Jordan). The Use of In Situ Hybridization Histochemistry to Study Gene Expression in Mouse Neurological Mutants (G.D. Frantz and A.J. Tobin). In Situ Hybridization as a Means of Studying the Role of Growth Factors, Oncogenes, and Proto-Oncogenes in the Nervous System (M. Murray). The Use of In Situ Transcription in the Study of Gene Expression (J. Eberwine, I. Zangger, R. Van Gelder, C. Evans, and L. Tecott). In Situ Hybridization Histochemistry at the Electron Microscopic Level (J.-J. Soghomonian). Quantitative Analysis of In Situ Hybridization Using Image Analysis (A.J. Smolen and P. Beaston-Wimmer). Appendix. Index.