The main purpose of the two volumes on Immunochemistry of the Extracellular Matrix is to describe state of the art methods, which have been proven to provide antibody reagents of defined specificity to collagens as well as other glycoproteins found in association within connective tissue. The isolation and purification of collagens, procollagens, and related connective tissue proteins are described for several tissues, tissue culture cells and species. Immunization with these collagens in laboratory animals yields antibodies with different characteristic specificities: to the procollagen extension fragments, the non-helical segments of the a-chains, to helical and denatured determinants.
Table of Contents
1. Chemical Properties of Collagen 2. Methods of Collagen Fractionation 3. Preparation of Type 1 and Type 3 Collagen, their PN Collagens and Aminoterminal Extension Peptides 4. Preparation of Type 2 Collagen 5. Isolation and Characterization of Pepsin Solubilized Basement Membrane Collagens (Type 4) from Human Placenta, Bovine Kidney Cortices, and Bovine Lens Capsule 6. The Preparation of Type V (AB2) Collagen 7. Preparation of Antisera Specific for the Amino and Carboxyl Propeptides of Type 1 and Type 2 Procollagen 8. Isolation of Fibronectin from Plasma and Cells 9. Laminin a Glycoprotein from basement membranes 10. Isolation and Properties of Chondronectin, the Chondrocyte Attachment Factor 11. Immunization Procedures, Isolation by Affinity Chromatography and Serological and Immunochemical Characterization of Collagen Specific Antibodies 12. Production of Monoclonal Antibodies to Collagen and their Immunofluorescence Localization in Embryonic Cornea and Cartilage 13. Radioimmunoassay (ELISA) for Connective Tissue Proteins: Type 1 Collagen.