1st Edition
Immunoassays Development, Applications and Future Trends
The concept behind this book is to provide a detailed and practical overview of the development and use of immunoassays in many different areas. Immunoassays are analytical tests that utilise antibodies to measure the amount, activity or identity of an analyte. This book is designed to provide a critical and helpful insight into the subject and to give the user practical information that may be of assistance in assay format selection, antibody generation/selection and choice of appropriate detection strategies. It is comprised of 13 chapters written by highly experienced researchers in the fields of antibody-based research, immunoassay development, assay validation, diagnostics and microfluidics.
Beginning with a comprehensive survey of antibodies, immunoassay formats and signalling systems, the book elucidates key topics related to the development of an ideal antibody-based sensor, focuses on the important topic of surface modification, explores key parameters in the immobilisation of antibodies onto solid surfaces, discusses the move to ‘lab-on-a-chip’-based devices and investigates the key parameters necessary for their development. Three of the chapters are dedicated to the areas of clinical diagnostics, infectious disease monitoring and food security, where immunoassay-based applications have become highly valuable tools. The future of immunoassays, including next-generation immunoassays, electrochemical-immunoassays and ‘lab-on-a-chip’-based systems, is also discussed. The book also covers the use of optical detection systems (with a focus on surface plasmon resonance) in immunoassays, provides a compilation of important, routinely used immunoassay protocols and addresses problems that may be encountered during assay development.
An Overview of Immunoassays
Caroline Murphy, Sarah Gilgunn, Richard O’Kennedy
Recombinant Antibodies for Diagnostic Applications: Design Considerations and Structural Correlates
Stephen Hearty, Richard O’Kennedy
Surfaces and Immobilisation Strategies for use in Immunoassay Development
Chandra K. Dixit, Paul Conroy
Immunoassay Validation
Paul Leonard
Lab-on-a-Chip Immunoassay Systems
Barry Byrne, Louise M. Barrett
Clinical Applications of Immunoassays
Karen Lawler, Gregor Kijanka
Immunoassay-Based Detection of Parasitic Diseases
Paul J. Conroy
Detection of Food, Agricultural and Aquatic Contaminants
Marie Le Berre, Caroline Viguier, Caroline Murphy, Niamh Gilmartin
Next-Generation Immunoassays
Valerie Fitzgerald, Paul Leonard
Recent Trends and the Future of Electrochemical Immunoassay Systems
Frank Davis, Seamus Higson
New Developments in Lab-on-a-Chip Immunoassay Devices
Louise M. Barrett, Barry Byrne
Optical Signal Transduction with an Emphasis on the Application of Surface Plasmon Resonance in Antibody Characterisation
Caroline Murphy, Aoife Crawley, Hannah Byrne, Robert Tallon, Kara Moran, Jenny Fitzgerald, Richard O’Kennedy
Protocols for Key Steps in the Development of an Immunoassay
Caroline Murphy, Richard O’Kennedy
Biography
O'Kennedy, Richard; Murphy, Caroline