Gastroenterology has advanced through the development and application of increasingly sophisticated methods to measure changes in gastrointestinal function. Handbook of
Methods in Gastrointestinal Pharmacology brings together details on commonly employed approaches in investigative gastroenterology.
The book provides comprehensive coverage of methods and techniques used to investigate the mechanism of action of drugs on the GI tract. An integral part of each chapter is the discussion of development of techniques based upon physiologic mechanisms and principles in pharmacology. In vivo and in situ techniques involving whole animals, isolated tissue methodology, the use of single cell systems, and molecular biology approaches are covered. Illustrations provide a clear understanding of methodologies discussed.
Emphasis is placed on advantages and disadvantages of each technique in answering specific research questions. Chapters are written by experts experienced in the techniques they discuss; many pioneered one or more widely used methods. The wide variety of topics included make the Handbook of Methods in Gastrointestinal Pharmacology useful to established investigators, research fellows, and graduate students. Additionally, reviewers of grants and manuscripts can use it to clarify questions that arise regarding appropriate use of a technique in a particular setting.
Table of Contents
Methods for Assessing Gastric Secretion, T. Shea-Donohue
Brain Control of Gastric Secretion: Experimental Methods of Approach, V. Martínez and Y. Taché
Gastroduodenal Mucosal Protection Studies, G.S. Smith, J.C. Barreto, and T.A. Miller
Intestinal Absorption and Secretion: In Vivo Studies, P.L. Smith
Assessment of Intestinal Electrolyte Transport In Vitro, S.M. O'Grady
Criteria for Defining Enteric Neurotransmitters, C.H.V. Hoyle and G. Burnstock
Enteric Neurotransmitters and Hormone Receptors, D.R. Brown
In Vivo Motility Techniques, N.W. Weisbrodt and P. Bass
In Vitro Techniques for the Study of Gastrointestinal Motility, W.H. Percy
Isolated Smooth Muscle Cells, J.R. Grider and K.S. Murthy
In Vitro Electrophysiological Methods in Gastrointestinal Pharmacology, J.J. Galligan
Gastrointestinal Sphincter Function, K.R. DeVault and S. Rattan
Assessment of CNS Control of Gastrointestinal Motility, L. Bueno
Evaluation of Antimotion Sickness Drugs, J.J. Stewart and C.D. Wood
Evaluation of Visceral Pain, G.F. Gebhart and J.N. Sengupta
The Gut as a Model of Opioid Dependence, T.F. Burks
Methods for Studying Splanchnic Blood Flow, K.D. Crissinger and D.N. Granger
Isolation and Characterization of Rat Intestinal Mucosal Mast Cells, E.Y. Bissonnette, B. Chin, and A.D. Befus
Molecular Biology Methods in Gastrointestinal Pharmacology, C.D. Ulrich, II and L.J. Miller
"The text is well edited, is timely, and nicely compiles the large number of laboratory techniques commonly used in gastrointestinal (GI) research...a must for established investigators in GI research and a highly recommended addition to the libraries of medical research institutions."
-J. Med. Chem.