1st Edition

Handbook of Dynein

Edited By Keiko Hirose Copyright 2012
    404 Pages 34 Color & 48 B/W Illustrations
    by Jenny Stanford Publishing

    This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students.

    Besides comprehensive cover of structural information gained by electron microscopy, electron cryo-tomography, X-ray crystallography, and nuclear magnetic resonance, this book provides detailed descriptions of mechanistic experiments by single-molecule nanometry. The reports include in vitro studies of the motility of reconstituted complexes and in vivo studies of organisms expressing mutant dyneins. The recent technical improvements described, which have played an important part in recent advances, include the expression and preparation of recombinant dynein heavy chains or individual subdomains.

    Dyneins: Ancient Protein Complexes Gradually Reveal Their Secrets, L. A. Amos and K. Hirose
    Two Decades of Cytoplasmic Dynein: From Fast to Forceful, R. J. McKenney and R. B. Vallee
    Functional Analysis of the Dynein Motor Domain, T. Shima, K. Sutoh, and T. Kon
    Structural Studies on the Dynein Heavy Chain, A. J. Roberts and S. A. Burgess
    Structural Analysis of Microtubule-Bound Dynein, K. Hirose
    The Dynein Stalk: Atomic Structure and Roles in the Mechanism of the Dynein Motor, A. P. Carter
    Enzymatic and Motile Properties of Native Dynein Molecules, Y. Toyoshima and H. Higuchi
    Motile Properties of Cytoplasmic Dynein, S. L. Reck-Peterson, R. D. Vale, and A. Gennerich
    Motile Properties of Inner Arm Dynein, Y. Shitaka, H. Kojima, and K. Oiwa
    Dynein Motility in Cilia and Flagella, A. Gokhale, M. Wirschell, W. Sale, and D. R. Mitchell
    3D Structures of Axonemes, T. Ishikawa
    Functional Diversity of Axonemal Dyneins, R. Kamiya
    Assembly and Regulation of Dynein Light Chains, A. Nyarko and E. Barbar
    Regulatory Subunits of Axonemal Dynein, K. Inaba
    The Role of Dynein in Yeast Nuclear Segregation, M. D. Stuchell-Brereton, J. K. Moore, and J. A. Cooper

    Biography

    Keiko Hirose

    "Given the recent explosion of interest in dynein, this book gives a timely review of both historical and current developments in the field. It is an extensive compendium compiled by leading dynein researchers and an excellent resource for young and experienced scientists alike!"
    —Prof. Joe Howard, Max Planck Institute of Molecular Cell Biology and Genetics, Germany

    "Handbook of Dynein, edited by the eminent electron microscopists Keiko Hirose and Linda Amos and contributed to by many international leading scientists in the field, is an excellent introduction to cutting-edge dynein research, including such aspects as biochemistry, molecular biology, biophysics, structural biology, and molecular genetics. It introduces not only the mechanisms of how cilia and flagella move and how the intracellular transport is performed by dyneins but also the pathogenesis of diseases related to the dynein motor complex. Thus, it will be a really fine handbook for students and researchers in the broad areas of life sciences."
    —Prof. Nobutaka Hirokawa, University of Tokyo, Japan