Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process.
Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps.
What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.
Table of Contents
Silver Stains for the Identification of Neuroendocrine Cell Types, L. Grimelius
Autometallographic Tracing of Gold, Silver, Bismuth, Mercury and Zinc, G. Danscher, G.W. Hacker, and M. Stoltenberg
Silver- and Gold-Based Autometallography of Nanogold, J.F. Hainfeld and R.D. Powell
Immunogold-Silver Staining for Light Microscopy Using Colloidal or Clustered Gold (Nanogold), G.W. Hacker, A.L.M. Cheung, R.R. Tubbs, L. Grimelius, G. Danscher, and C. Hauser-Kronberger
The Protein A-Gold-Silver Staining Method, O. Fujimori
Microscopic Uses of Nanogold, J.F. Hainfeld, R.D. Powell, and F.R. Furuya
Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations, R.D. Powell and J.F. Hainfeld
Detection of Human Papillomavirus by In Situ Hybridization Using an In Situ PCR Thermal Cycler, P. Jackson and F.A. Lewis
Super Sensitive In Situ Hybridization by Tyramide Signal Amplification and Nanogold Silver Staining: The Contribution of Autometallography and Catalyzed Reporter Deposition to the Rejuvenation of In Situ Hybridization, R.R. Tubbs, J. Pettay, T. Grogan, A.L.M. Cheung, R. Powell, J. Hainfeld, C. Hauser-Kronberger, and G.W. Hacker
Immunogold Labeling Techniques for Transmission Electron Microscopy: Applications in Cell and Molecular Biology, L. Lossi, P. Aimar, E. Beltramo, and A. Merighi
Pre-embedding Immunoelectron Microscopy with Nanogold Immunolabeling, Silver Enhancement, and Its Stabilization by Gold, H. Sawada and M. Esaki
Gold Cluster Immunoprobes: Light and Electron Microscopy, J.M. Robinson, T. Takizawa, and D.D. Vandré
Highly Sensitive Ultrastructural Immunogold Detection Using Tyramide and Gold Enhancement, C. Schöfer and K. Weipoltshammer
In Situ Hybridization at the Electron Microscopic Level, C. Schöfer and K. Weipoltshammer
Immunogold-Silver Staining for Scanning Electron Microscopy in Cancer Research, B.-L. Wang, N. Flay, and G.W. Hacker
Immunogold-Silver Scanning Electron Microscopy Using Glycerol Liquid Substitution, J. Samaj, H.-J. Ensikat, W. Barthlott, and D. Volkmann
"The book is a timely presentation of all the latest techniques in gold and silver staining. I would recommend the book to all pathologists, microscopists, and especially molecular morphologists."
- Shashi Singh, Centre for Cellular and Molecular Biology, in Microscopy and Analysis