While mice have always been highly popular laboratory subjects, their suitability for genetic engineering has solidified their position as today's lab animal model of choice. However, their increased use in genetic studies has created a demand for input on phenotyping that is not always easily met.
To improve the flow of information on the pathology of mice with spontaneous or genetically engineered mutations, prominent researchers organized a series of meetings. Recognizing other needs, the organizers gradually broadened their focus, until finally they expanded to provide an overview of the entire field of genetically engineered models.
The Genetically Engineered Mice Handbook is an extension of those meetings. It offers an introduction for those entering into this area of research, while also serving as a resource for those presently employing mice as laboratory models. Highly comprehensive, this volume covers pertinent aspects of genetically engineered mice, including the use of models for developmental biology and the monitoring of laboratory colonies. With contributions from nearly five-dozen leading researchers, the text presents systematic approaches for analyzing mutant mice for specific medical applications, details a variety of methods for creating mutants and includes information that is particularly hard to access dealing with legal responsibilities.
This essential reference examines commonly used traditional, as well as emerging, technologies
To address the purpose of the original meeting, the Genetically Engineered Mice Handbook directs researchers to the best public websites, and offers instruction on how to use them. In the past, as their work dictated, researchers would seek out experts on particular organ systems. Now groups of experts work together to generate these websites, providing the latest data as well as discussions over points of debate. These sites do not eliminate the need for a trained pathologist, but they do provide reference materials for those lacking expertise in particular anatomic structures. They also offer much greater numbers of examples than are available in print, from which biomedical researchers can draw.
Table of Contents
Genetically Engineered Mice: Past, Present, and Future: John P. Sundberg, Tsutomu Ichiki, Jerrold M. Ward, and Björn Rozell
Sharing Reseach Tools: The Laboratory Mouse: David Einhorn
Managing Success: Mutant Mouse Repositories: Stephen F. Rockwood, Martin D. Fray, and Naomi Nakagata
Mouse Genome Informatics: Database Access to Integrated Phenotype Data: Carroll-Ann W. Goldsmith, Martin Ringwald, John P. Sundberg, Carol J. Bult, and Janan T. Eppig
Genetic Resouce Databases in Japan: Yukiko Yamazaki
Computational Pathology: Challenges in the Informatics of Phenotype Description in Mutant Mice: Paul N. Schofield, Jonathan B. L. Bard, Björn Rozell, and John P. Sundberg
Biological Methods for Archiving and Maintaining
Mutant Laboratory Mice: Martin D. Fray, Peter H. Glenister, Steven Rockwood, Takehito Kaneko, and Naomi Nakagata
Mouse Genetic Resources without Germ Cells: Somatic Cell Nuclear Transfer and ES Technology: Teruhiko Wakayama
The Present Status of Somatic Cell Cloning: Atsuo Ogura, Kimiko Inoue, Narumi Ogonuki, and Hiromi Miki
Exchangeable Gene Trapping: Kimi Araki
Genetic Monitoring of Mice: Hideki Katoh
Microbiological Monitoring of Laboratory Mice: James R. Fahey
Effect of Intestinal Flora on Phenotype: Seiko Narushima and Kikuji Itoh
Helicobacter pylori and Stomach Cancer: Masae Tatematsu, Tetsuya Tsukamoto, and Tsutomu Mizoshita
Professional Use of Mutant Laboratory Mice in Research: John P. Sundberg and Carol J. Bult
Phenotyping Postpartum Mutant Laboratory Mice and Determining Their Value for Human Diseaes: John P. Sundberg and Tsutomu Ichiki
Common Diseases Found in Inbred Strains of Laboratory Mice: John P. Sundberg and Tsutomu Ichiki
Colon Cancer and Polyposis Models: Makoto Mark Taketo
Phenotypic Analysis of Mice with Steroid Deficiency: Noomen Ben El Hadj, Meng-Chun Hu, Hsueh-Ping Chu, Leo Chi-Kuang Wang, and Bon-chu Chung
External Genitalia Development: A Model System to Study Organogenesis: K. Suzuki, H. Nishida, S. Ohta, Y. Satoh, Y. Xu, Y. Zhang, Y. Wada, Y. Ogino, N. Nakagata, T. Ohba, and G. Yamada
Genetic Approaches to Investigate Retinoic Acid Functions in Mouse Development: Pascal Dollé, Karen Niederreither, Julien Vermot, Vanessa
Ribes, Jabier Gallego Llamas, and Isabelle Le Roux
Mouse Models for Developmental Biology: Functional Analysis of Ror and Wnt Signaling: Isao Oishi, Akinori Yoda, Shuichi Kani, and Yasuhiro Minami
"…I found an oasis of practical information making this book well worth obtaining. …clear and concise …descriptions of cryopreservation techniques for embryos, gametes, and ovarian tissue. Everything you need to know was laid out in outline form with illustrations and references."
--Mark Klinger for Laboratory Animal Practitioner, Vol. 39, No. 1, March 2006
"The Genetically Engineered Mice Handbook effectively summarizes commonly used and emerging technologies to generate, characterize, and conduct research on genetically engineered mice . . . provides a broad multinational perspective on the use of genetically engineered mice for functional genomics research . . . well organized and highly informative. . ."
– Dr. Cory Brayton, School of Medicine, Johns Hopkins University, in Veterinary Pathology, 2007, Vol. 44, No. 4