Jim Mcwhir, Alison Thomson
Taylor & Francis
Published July 1, 2004
Reference - 216 Pages
ISBN 9781859963609 - CAT# RU39607
Series: Advanced Methods
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Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.
Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.