Lipids play an essential role in cell signaling and subcellular structure. Systematic analysis of the total lipid structure of a cell or organism, the lipodome, can reveal novel avenues of therapeutic intervention and diagnosis. This analysis is best modeled after the lessons learned from proteomics. With contributions from pioneering researchers from the US, Europe, Canada, Japan, and China, Functional Lipidomics
introduces lipidomics, explores the current state of the field, and provides a comprehensive background for future groundbreaking work in this burgeoning field.
The first book dedicated to the topic, Functional Lipidomics first defines and introduces the field before delving deeply into the methods, processes, analyses, and research aspects. The book uncovers the biosynthesis, metabolism, and function of fatty acids, acylglyerols, glycerophospholipids, sphingolipids, terpenes, and lipids that complex with proteins. Taking both academic and industrial perspectives, the contributors elaborate upon cutting-edge approaches to lipid analysis, diagnostic approaches using lipid and lipid-binding protein patterns, and the therapeutic significance of targeting proteins involved with the lipid signaling pathway.
Functional Lipidomics elucidates state-of-the-art strategies and techniques that enable us to understand biochemical patterns related to disease states, develop new targets for drug design, and identify important markers of a developmental stage, pathological condition, or disease treatment.
Table of Contents
The LIPID MAPS Approach to Lipidomics
Edward A. Dennis, H. Alex Brown, Raymond A. Deems, Christopher K. Glass, Alfred H. Merrill, Jr., Robert C. Murphy, Christian R.H. Raetz, Walter Shaw, Shankar Subramaniam, David W. Russell, Michael S. VanNieuwenhze, Stephen H. White, Joseph L. Witztum, and John Wooley
LC/MS Methodology in Lipid Analysis and Structural Characterization of Novel Lipid Species
Robert C. Murphy, Jessica Krank, and Robert M. Barkley
Functional Plasticity of Lipid Mediators: The Example of Endocannabinoids
Luciano De Petrocellis, Mario van der Stelt, and Vincenzo Di Marzo
Rebecca C. Bowers-Gentry, Raymond A. Deems, Richard Harkewicz, and Edward A. Dennis
Functional Lipidomics: Lysophosphatidic Acid as a Target for Molecular Diagnosis and Therapy of Ovarian Cancer
Janos L. Tanyi, David Crotzer, Judith Wolf, Shuangxing Yu, Yutaka Hasegawa, John Lahad, Kwai Wa Cheng, Makiko Umezu-Goto, Glenn D. Prestwich, Andrew Morris, Robert A. Newman, Edward A. Felix, Rose Lapis, and Gordon B. Mills
Analysis of Lysophospholipids in Human Body Fluids: Comparison of the Lysophospholipid Content in Malignant vs. Nonmalignant Diseases
Yi Jin Xiao and Yan Xu
Functional Lipidomics: Lessons and Examples from the Sphingolipids
Yusuf A. Hannun
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Analysis of Sphingolipids
M. Cameron Sullards and Alfred H. Merrill, Jr.
Methods of Probing Phosphoinositides–Protein Interactions
Li Feng, Colin Ferguson, Paul O. Neilsen, Leena Chakravarty, Piotr W. Rzepecki, and Glenn D. Prestwich
Fishing for Pharmaceutically Relevant phosphoinositide-Binding Proteins Using Chemical Proteomics
Christian Pasquali and Christian Rommel
Phosphoinositide Proﬁling in Complex Lipid Mixtures
Markus R. Wenk and Pietro De Camilli
Multiplexed Lipid Arrays of Antiimmunoglobulin M–Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells
Stephen B. Milne, Jeffrey S. Forrester, Pavlina T. Ivanova, Michelle D. Armstrong, and H. Alex Brown
Specific Lipid Alterations in Alzheimer’s Disease and Diabetes: Shotgun Global Cellular Lipidome Analyses by Electrospray Ionization Mass Spectrometry Using Intrasource Separation
Xianlin Han and Richard W. Gross
High-Throughput Lipid Profiling to Identify and Characterize Genes Involved in Lipid Metabolism, Signaling, and Stress Response
Ruth Welti, Jyoti Shah, Steven LeVine, Wynn Esch, Todd Williams, and Xuemin Wang