This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.
Table of Contents
1. Freeze-Fracture Images: 1967-1971 2. Membrane-Splitting Analyses of Membrane-spanning Proteins 3. Labelling Intramembrane Particles in Freeze-Fracture Replicas 4. Distribution and Mobility of Receptors in the Plasma Membrane 5. Freeze-Fracture Sterol Localisation with Filipin 6. Polymorphic Phase Behaviour of Membrane Lipids 7. The Kinetics of Artificially Induced Membrane Fusion 8. Freeze-Fracture (-Etching) Analysis of Exo-Endocytosis 9. The Vitelline Layer and Fertilization Envelope of Echinoderm and Amphibian Eggs: Visualisation of a Cell Surface-Anchored Extracellular Matrix 10. The Plasma Membrane of Spermatozoon