1st Edition

Forensic Applications of High Performance Liquid Chromatography

    276 Pages 12 Color & 149 B/W Illustrations
    by CRC Press

    276 Pages
    by CRC Press

    Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective.

    Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines:

    • The history of HPLC and the theory behind the separation process
    • The requirements for successful analysis and best practice tips
    • The modes of separation and detection most appropriate for forensic science applications
    • HPLC method development and evaluation
    • The quality aspects of laboratory operation
    • Troubleshooting HPLC systems and analyses
    • Applications of HPLC within the field of forensic science

    Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers.

    An instructor’s manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption

    Introduction to HPLC

    Introduction

    The History of Chromatography

    Basic Instrumentation

    Further Reading

    Basic Principles of HPLC

    Theory of Chromatography

    Chemical Bonding and Polarity

    Mobile Phase Preparation, the Use of Buffers, and Sample Preparation

    Mobile Phase Preparation

    Buffers

    Sample and Standard Preparation

    Modes of Separation

    Introduction

    Reverse Phase HPLC

    Ion Exchange

    Normal Phase HPLC

    Detection Systems

    Introduction

    Theory

    UV/Vis Detectors versus Diode Array Detectors

    Electrochemical Detectors

    Mass Spectrometry

    Method Development in Reversed Phase HPLC

    Introduction

    Sample Composition

    Which Column Will Affect the Separation?

    Choosing a Mobile Phase

    Automated Method Development

    Method Development Strategy

    Method Development Worked Example

    System Suitability

    Introduction

    System Suitability

    System Performance

    Chromatographic Suitability

    Qualification, Validation, and Verification

    Introduction

    Valid Analytical Measurement

    Estimation of Uncertainty of Measurement, Sources of Error, and Statistical Evaluation

    Instrument Qualification

    Method Validation

    Method Verification

    Quality Systems

    Introduction

    What Is Quality?

    Introduction to Quality Assurance

    How Do We Ensure Quality in Our Laboratories?

    A Quality Management System

    Accreditation

    Auditing

    Troubleshooting HPLC Systems

    Introduction

    Troubleshooting

    Tool Kit and Spare Parts

    Preventative Maintenance

    Column Care

    Forensic Applications of HPLC

    Introduction

    Drug Analysis

    Toxicological Analysis

    Colour Analysis

    Analysis of Explosives

    Food and the Environment

    Glossary

    Index

    Biography

    Shirley Bayne, Michelle Carlin

    I showed this book to many of my students and asked them to read it and they told me that they could understand the concepts of HPLC properly for the first time from this book. Even a number of my own concepts were clarified a great deal. … This is the first book to the best of my knowledge specifically dealing with the forensic applications of HPLC, and a very good one at that. Highly recommended for all forensic scientists and chemists, especially those who are working in separation science.
    — Anil Aggrawal writing in Anil Aggrawal's Internet Journal of Forensic Medicine and Toxicology

    "
    … easy to read and lively enough to engage students and keep their interest."
    — C. F. Poole, Wayne State University, in Chromatographia

    "The book chapters maintain a balance between theoretical considerations and examples and practical solutions. … designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is an invaluable guide for those in the early stages of their forensic analysis careers."
    International Journal of Environmental Analytical Chemistry