Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics

Laura Marcu, Paul M. W. French, Daniel S. Elson

July 17, 2014 by CRC Press
Reference - 570 Pages - 240 Color Illustrations
ISBN 9781439861677 - CAT# K12851

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  • Provides a comprehensive treatment of fluorescence lifetime spectroscopy and imaging using both endogenous and exogenous probes
  • Discusses the fundamentals, state-of-the-art techniques, and their current applications in tissue diagnostics for research and clinical applications
  • Written by the leading experts in this field, which has advanced as a result of tremendous progress in optical and electronics technologies, including pulsed lasers and fast electronics, detectors and imaging cameras
  • Offers a survey of instrumentation and analysis of fluorescence lifetime data, including global analysis and computational approaches
  • Covers the wide range of clinical applications in oncology, cardiovascular, metabolism, dermatology, tissue engineering, and ophthalmology, as well as photodynamic therapy and tomographic FLIM of animals


During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications.

Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation.

Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.