1st Edition
Effect of Sulphide on Enhanced Biological Phosphorus Removal
The enhanced biological removal of phosphorus (EBPR) is a popular process due to high removal efficiency, low operational costs, and the possibility of phosphorus recovery. Nevertheless, the stability of the EBPR depends on different factors such as: temperature, pH, and the presence of toxic compounds. While extensive studies have researched the effects of temperature and pH on EBPR systems, little is known about the effects of different toxic compounds on EBPR. For example, sulphide has shown to inhibit different microbial activities in the WWTP, but the knowledge about its effects on EBPR is limited. Whereas the sulphide generated in the sewage can cause a shock effect on EBPR, the continuously exposure to sulphide potentially generated in WWTP can cause the acclimatization and adaptation of the biomass.
This research suggests that sulphate reducing bacteria can proliferate in WWTP, as they are reversibly inhibited by the recirculation of sludge through anaerobic-anoxic-oxic conditions. The research enhances the understanding of the effect of sulphide on the anaerobic-oxic metabolism of PAO. It suggests that the filamentous bacteria Thiothrix caldifontis could play an important role in the biological removal of phosphorus. It questions the ability of PAO to generate energy from nitrate respiration and its use for the anoxic phosphorus uptake. Thus, the results obtained in this research can be used to understand the stability of the EBPR process under anaerobic-anoxic-oxic conditions, especially when exposed to the presence of sulphide.
1 Introduction
1.1. Background
1.2. Scope of the thesis
1.3. Literature Review
1.4. Problem statement and objectives
1.5. Research approach
1.6. Outline
2 Effect of electron acceptors on sulphate reduction activity at WWTP
2.1. Highlights
2.2. Abstract
2.3. Introduction
2.4. Material and methods
2.5. Results
2.6. Discussion
2.7. Conclusion
3 Sulphide effects on the physiology of Candidatus Accumulibacter phosphatis Type I
3.1. Highlights
3.2. Abstract
3.3. Introduction
3.4. Materials and methods
3.5. Results
3.6. Discussion
3.7. Conclusions
4 Long-term effects of sulphide on the enhanced biological removal of phosphorus: The symbiotic role of Thiothrix caldifontis
4.1. Highlights
4.2. Graphical Abstract
4.3. Abstract
4.3. Introduction
4.4. Materials and methods
4.5. Results
4.6. Discussion
4.7. Conclusions
5 Cooperation between Competibacter sp. and Accumulibacter in denitrification and phosphate removal processes
5.1. Highlights
5.2. Abstract
5.3. Introduction
5.4. Materials and Methods
5.5. Results
5.6. Discussion
5.7. Conclusions
6 Absent anoxic activity of PAO I on nitrate under different long-term operational conditions
6.1. Highlights
6.2. Abstract
6.3. Introduction
6.4. Material and methods
6.5. Results
6.6. Discussion
6.7. Conclusions
7 Outlook and main conclusions
7.1. General conclusions
7.2. Specific conclusions
7.3. Evaluation and outlook
References
Annex
Effect of electron acceptors on sulphate reduction activity at WWTP
Long-term effects of sulphide on the enhanced biological removal of phosphorus: The role of Thiothrix caldifontis
Cooperation between Competibacter sp. and Accumulibacter in denitrification and phosphate removal processes
Absent anoxic activity of PAO I on nitrate under different long-term operational conditions
Biography
Francisco Rubio was born in 1986 in Durango, México. With a background in civil engineering, Francisco gained work experience in different companies and projects throughout México and abroad. He decided to obtain further education in the field of Waste Water treatment and started his MSc in Municipal Water and Infrastructure, specialization Sanitary Engineering at UNESCO-IHE in October 2011, where he graduated with distinction in April 2013. His research focus was on the recovery of nitrogen and phosphorous (as Struvite) from waste streams, using affordable sources of magnesium such as Seawater.
