This book covers the principles of cryopreservation as they relate the preservation of viable cells and cell materials being developed for biopharmaceutical applications. Topics include: the principles of freezing and thawing cells, physiochemical phenomena, process and system design options, method selection considerations, preservation procedures, cryoprotectant additives, freeze-drying human live virus vaccines, and transport system selection criteria. Contributions from well-known experts such as Steven S. Lee, Thomas C. Pringle, William H. Siegel, Richard Wisniewski, and Fangdong Yin make this the single most important study available.
Table of Contents
Principles & Scale-up: Cryopreservation for Cells, Microorganisms, Protein Solutions, Biopharmaceutical Formulations, & Biological Products, R. Wisniewski
Large-Scale Cryopreservation: Process Development for Freezing & Thawing of Large Volumes of Cell Suspensions, Protein Solutions & Biological Products, R. Wisniewski
Methods: Long-Term Preservation of Mammalian Cells & Microorganisms, C. Wagner
Cryopreservation of Mammalian Cell Culture Materials, W. Siegel
Freeze-Drying of Human Live Virus Vaccines, S. Lee & F. Yin
The Design & Use of Thermal Transport Containers, T. Pringle